LOCUS       sequence-64795-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGBKCg
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(68..115)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(155..184)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(203..222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(204..222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(233..673)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    687..811
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(954..973)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cccggaatta gcttggctgc
       61 aagcgcgcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatg
      121 cggccgctgc aaattcggcc tccatggcca tatgcaggtc ctcctctgag atcagcttct
      181 gctcctccat gatggcggct cgccctatag tgagtcgtat tacaaattcc ggcgatacag
      241 tcaactgtct ttgacctttg ttactactct cttccgatga tgatgtcgca cttattctat
      301 gctgtctcaa tgttagaggc atatcagtct ccactgaagc caatctatct gtgacggcat
      361 ctttattcac attatcttgt acaaataatc ctgttaacaa tgcttttata tcctgtaaag
      421 aatccatttt caaaatcatg tcaaggtctt ctcgaggaaa aatcagtaga aatagctgtt
      481 ccagtctttc tagccttgat tccacttctg tcagatgtgc cctagtcagc ggagaccttt
      541 tggttttggg agagtagcga cactcccagt tgttcttcag acacttggcg cacttcggtt
      601 tttctttgga gcacttgagc tttttaagtc ggcaaatatc gcatgcttgt tcgatagaag
      661 acagtagctt catcttggta ccatcaacca ctttgtacaa gaaagctgaa cgagaaacgt
      721 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      781 actgtaaaac acaacatatc cagtcactat ggtcgacctg cagactggct gtgtataagg
      841 gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
      901 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
      961 gtggtcatca tgcgccagct ttcatccccg a
//
