LOCUS       sequence-64797-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGBKCg
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(131..255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             269..709
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     720..739
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        720..738
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             758..787
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      827..874
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
       61 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
      121 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
      241 cttgtacaaa gtggttgatg gtaccaagat gaagctactg tcttctatcg aacaagcatg
      301 cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg
      361 tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag
      421 ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat
      481 ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc
      541 attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt
      601 ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc
      661 atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcgc cggaatttgt
      721 aatacgactc actatagggc gagccgccat catggaggag cagaagctga tctcagagga
      781 ggacctgcat atggccatgg aggccgaatt tgcagcggcc gcataactag cataacccct
      841 tggggcctct aaacgggtct tgaggggttt tttgcgcgct tgcagccaag ctaattccgg
      901 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa a
//
