LOCUS       sequence-64798-e       14000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Finney-Manchester SP, Maheshri N
  TITLE     Harnessing mutagenic homologous recombination for targeted 
            mutagenesis in vivo by TaGTEAM.
  JOURNAL   Nucleic Acids Res. 2013 Mar 7.
  PUBMED    23470991
REFERENCE   2  (bases 1 to 14000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Mar 7."
COMMENT     Sequence Label: PRS303-240xtetO
FEATURES             Location/Qualifiers
     source          1..14000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1165
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1426..1881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1563..1584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1775..1794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2011..2033
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2025..2042
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2026..2042
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2049..2068
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2049..2067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2695..2713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2724..2742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2858..2876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2934..2952
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2963..2981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3039..3057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3068..3086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3115..3133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3144..3162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3249..3267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3364..3382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3411..3429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3440..3458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3469..3487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3541..3559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3570..3588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3617..3635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3646..3664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3675..3693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3722..3740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3751..3769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3780..3798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3827..3845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3883..3901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3930..3948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3959..3977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3988..4006
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4035..4053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4064..4082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4093..4111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4140..4158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4169..4187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4198..4216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4245..4263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4274..4292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4348..4366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4377..4395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4406..4424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4453..4471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4482..4500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4511..4529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4586..4604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4661..4679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4690..4708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4719..4737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4766..4784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4795..4813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4824..4842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4871..4889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4900..4918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4929..4947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4976..4994
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5005..5023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5099..5117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5128..5146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5174..5192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5203..5221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5232..5250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5307..5325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5336..5354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5383..5401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5486..5504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5515..5533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5544..5562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5591..5609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5620..5638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5649..5667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5696..5714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5725..5743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5754..5772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5821..5839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5998..6016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6027..6045
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6056..6074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6103..6121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6160..6178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6207..6225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6264..6282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6311..6329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6340..6358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6368..6386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6415..6433
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6444..6462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6519..6537
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6548..6566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6623..6641
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6652..6670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6758..6776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(6824..7357)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    7610..7628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7639..7657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7686..7704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7744..7762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7791..7809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7820..7838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7849..7867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7895..7913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7924..7942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7953..7971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8000..8018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8029..8047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8105..8123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8134..8152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8162..8180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8209..8227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8238..8256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8267..8285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8314..8332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8343..8361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8372..8390
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8419..8437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8448..8466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8477..8495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8582..8600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8629..8647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8658..8676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8732..8750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8761..8779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8864..8882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8893..8911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8940..8958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8969..8987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8998..9016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9045..9063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9074..9092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9103..9121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9150..9168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9179..9197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9208..9226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9284..9302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9360..9378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9402..9420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9449..9467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9506..9524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9554..9572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9612..9630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9659..9677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9688..9706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9717..9735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9763..9781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9792..9810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9821..9839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9868..9886
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9926..9944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9973..9991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10002..10020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10031..10049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10078..10096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10182..10200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10212..10230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10241..10259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10288..10306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10317..10335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10451..10469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10527..10545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10811..10829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10840..10858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10869..10887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10916..10934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10945..10963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10974..10992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11021..11039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11050..11068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11119..11137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11148..11166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11177..11195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11224..11242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11281..11299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11328..11346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11357..11375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11385..11403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11432..11450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11461..11479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11490..11508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11566..11584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11628..11646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11657..11675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(11750..11768)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11789..11805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11789..11805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11802..11824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11813..11829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11837..11843,11844..11861,11862..11867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(12020..12037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12191..12779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(12950..13741,13742..13810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13573..13592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13811..13915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13973..13991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg ccgtgcgtgg
      841 agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt
      901 agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg
      961 agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat
     1021 taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt
     1081 caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc
     1141 ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat
     1201 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     1261 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     1321 ttcctttttt ctttttgctt tttctttttt tttctcttga actcgacgga tcatatgcgg
     1381 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta
     1441 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1501 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1561 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1621 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1681 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1741 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1801 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1861 atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag
     1921 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaagggggga tgtgctgcaa
     1981 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2041 gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct
     2101 ttcaagtagt tcacaaagag actcaaactg agatcatgga gacgtcggca agcaattgat
     2161 tgttagttgt tatcaaaact cattagcttc ggtaaaacta catagattga aaatcagtag
     2221 taacatacaa cgtcgtttgc atggggttac aaaccgacca acctgatgct ttcaaagatt
     2281 ctttacttac tggttcacag tggccaagaa gcatataaat ctgtactttg cagtaaaaaa
     2341 cagatgatat tgctcattcc cagcgattgg gcgcgccatt ttgacatata ctgatatgga
     2401 cctcttgttt cgtataaatc gctatttatt tccccagata actaaagaaa atccttcaac
     2461 ccagcgttct atattactat attctcaacc cgccgttttc ctgctgtgcg ataattctca
     2521 ttcaatacta ccatttcaaa cctagaaaag ggtgtcttta ttaaaactgt aagaaaattt
     2581 tatcatgaac tctgatgaaa agtcttcgga agatcaactt tttgattttt caaaaatgaa
     2641 agaaactgtt gtctccattt atggcctcat atctgaatct agagtacgtc cgtctctcta
     2701 tcactgatag ggattgctag atttctctat cactgatagg gacacgtcgc cctctctatc
     2761 actgataggg atggccggcc gtgctagagg ggcggtgctt ctctatcact gatagggact
     2821 ttcactcttc tctatcactg atagggatga tcaacgttct ctatcactga tagggatccc
     2881 cactcttgct agagtggtct cacctctcta tcactgacag ggacctctct tcctctctat
     2941 cactgatagg gacccttccc tctctctatc actgataggg accccaccca ctgctagagt
     3001 ttggctccgt ctctatcact gatagggatc tttcgtcatc tctatcactg atagggagcc
     3061 tcgcctttct ctatcactga tagggataat atgcattgct agagcacctg cgtttctcta
     3121 tcactgatag ggaaccgcga ctctctctat cactgatagg gatcgtcccc agtctctatc
     3181 actgataggg actgcctccg ctgctagaga cccccttgct ctctatcaca gatagggatt
     3241 tcgtgttctc tctatcactg atagggaccg ctcaccttct ctataactga tagggaaccc
     3301 gggccttgct agagccactt cttctccttt cactgatagg gaggcgctgc attctccgtg
     3361 ctgtctctat cactgatagg gatcccggcc tttgctagag ttggtatatc tctctatcac
     3421 tgatagggac ttctattctt ctctatcact gatagggatg gttccgcttc tctatcactg
     3481 atagggaccc tgctctgcta gagcttcttg cggtctatca ctgataggga gatgcacagc
     3541 tctctatcac tgatagggac gaccttgggt ctctatcact gatagggacc tgtcattctg
     3601 ctagagctcc ctgcattctc tatcactgat agggacagcg ctgagtctct atcactgata
     3661 gggatctcac cttttctcta tcactgatag ggattgatct tcttgctaga ggacccgtgt
     3721 ttctctatca ctgataggga cctctgattc tctctatcac tgatagggag gctcggggtt
     3781 ctctatcact gatagggact tgtcacgatg ctagagtcgg gacctgtctc tatcactgat
     3841 agggacccct gcctctctct atcactgata gggtctgcgt gctctctatc actgataggg
     3901 ataccatgac gtgctagagc cctgcgcgct ctctatcact gatagggatc catgctcttc
     3961 tctatcactg atagggatta cgatccctct ctatcactga tagggaccag ggtttgtgct
     4021 agagtgctgn agcgtctcta tcactgatag ggattttcct gtctctctat cactgatagg
     4081 gaatctcccc cctctctatc actgataggg agcttctgct atgctagagt ttagacccat
     4141 ctctatcact gatagggaac gatcgatttc tctatcactg atagggactt ccttgcctct
     4201 ctatcactga tagggagccc gcggagtgct agaggcaccc cctctctcta tcactgatag
     4261 ggagcgtgac ggctctctat cactgatagg gacctatatt ctctctatca ctgataagga
     4321 ccctcccagt gctagagttc ctttttctct ctatcactga tagggatggc ccctactctc
     4381 tatcactgat agggagcccg tccgctctct atcactgata gggagcctca tgcttgctag
     4441 agattgtctg tatctctatc actgataggg agtgttacgc ctctctatca ctgataggga
     4501 tggcctgtgt tctctatcac tgatagggat ccatcccact gctagagcga tgtcccctct
     4561 ctaccactga tagggactca tatcgtctct atcactgata gggacttatc tcgtctctat
     4621 cactcatagg gattcccctc gatgctagag gcgcccacac tctctatcac tgatagggaa
     4681 cgtgaatgtt ctctatcact gatagggaaa ttctttagtc tctatcactg atagggatcc
     4741 gcacttctgc tagagttcaa acgtgtctct atcactgata gggaccgtga tgcgtctcta
     4801 tcactgatag ggagtcttaa ctctctctat cactgatagg gactctcccg tttgctagag
     4861 ccctctgaga tctctatcac tgatagggag ctctgtagct ctctatcact gatagggatt
     4921 ttcccgcttc tctatcactg atagggagca ctttacgtgc tagagtcctg cgtgctctct
     4981 atcactgata gggagcccct ctcctctcta tcactgatag ggaacccgta ttttctctat
     5041 caagggagcc cattgactgc tagagatttc gtccttctct atcactgagt cgcttttctc
     5101 tctatcactg atagggaagt cctttcttct ctatcactga tagggagtaa acctctgcta
     5161 gagcatgttt gcctctctat cactgatagg gaacgctggc tttctctatc actgataggg
     5221 attcacgcac ctctctatca ctgataggga ggttcgcttt tgctagagcc tctatgtatc
     5281 tctatcactg ataggaggcc ctcccgtctc tatcactgat agggatattc accgatctct
     5341 atcactgata gggatcctta cgcgtgctag agctgccctg tctctctatc actgataggg
     5401 acttgcgctc tctctancac tgntaggnat gctttcttgt ctctatcact gataggaact
     5461 cttagtctgc tagagttccg atcgctctct atcactgata gggatccatc cttttctcta
     5521 tcactgatag ggacctcccc ttctctctat cactgatagg gatggacctg cttgctagag
     5581 ctggacctcg tctctatcac tgatagggat gcgccccact ctctatcact gatagggatc
     5641 ctctcctttc tctatcactg atagggaatc gcttcgctgc tagagtccaa cgctttctct
     5701 atcactgata gggatgtatc cccctctcta tcactgatag ggagtgcctc tgatctctat
     5761 cactgatagg gactcctctc tttgctagag ccgctcctca ctgataggga gtcattcgcc
     5821 tctctatcac tgatagggac ttttcagctt ctctaccact gatagggaac ttttccggtg
     5881 ctagagttga atccatctct atcactatag ggatcatgct ttggctctat cactgatagg
     5941 gatacttatt cttctctatc acggataggg acccttccct gctagagact ctatttttct
     6001 ctatcactga tagggactga taaccctctc tatcactgat agggagctcc tcacctctct
     6061 atcactgata gggacttgat tttctgctag agcatgccat tttctctatc actgataggg
     6121 atttgcccag ttctctatac tgatagggac tggctatcct ctctatcact gatagggacc
     6181 tgtatcgttg ctagagtgag tccccttctc tatcactgat agggattccc ctcgctctct
     6241 atcactgata gggcctcccc ctatctctat cactgatagg gacgtacccc cttgctagag
     6301 ctcttctccc tctctatcac tgatagggat tcctctctat ctctatcact gatagggaac
     6361 tacatactct ctatcactga tagggacccc caactctgct agaggccgct atgatctcta
     6421 tcactgatag ggattgccat atttctctat cactgatagg gaattctgat cctctctatc
     6481 actgatgggg attccgtctc tgctagagcc tctgccattc tctatcactg atagggaact
     6541 acttccttct ctatcactga tagggaccac tggatttctc tatcctgata gggacttcaa
     6601 agcctgctag agtctactgc gttctctatc actgataggg atccgtttta ctctctatca
     6661 ctgataggga ttccacacct ccctctatca ctgataggga ctctcgtctt tgctagagcg
     6721 tattaccttc tccatcactg atagggaccc cctttagtct ctatcactga tagggatcca
     6781 actccatctc tatcacgtga tagggacccc cgccagtgct agattaggtg gcggtacttg
     6841 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     6901 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     6961 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     7021 ctgcgagatc atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag
     7081 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     7141 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     7201 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     7261 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     7321 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     7381 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     7441 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     7501 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     7561 caggcgctag atagtctctc atctctatca cgatagggaa gtgcccctgt ctctatcact
     7621 gatagggaac ttcttgtctc tctatcactg atagggaccg cattttttgc tagaggcccc
     7681 catcttctct atcactgata gggaatgcgc cctctctcta tcactggtag ggaccccccc
     7741 ttttctctat cactgatagg gatctttgcg cgtgctagag attcccccgc tctctatcac
     7801 tgatagggat tcctcccctt ctctatcact gatagggacc tgctgcgttc tctatcactg
     7861 atagggagca cccgcgtgct agagtgccag tacctctcta tcactgatag ggaattgtcc
     7921 ctttctctat cactgatagg gaagtttttc catctctatc actgataggg atgtcatgcc
     7981 ttgctagagt ctatatttat ctctatcact gatagggagt ccccctcatc tctatcactg
     8041 atagggatac gtcccgatct ctatcactga tagggtctcg ctctcttgct agagtgcccg
     8101 gttttctcta tcactgatag ggacggaacg ccgtctctat cactgatagg gacgctatct
     8161 ctctctatca ctgataggga cccccatgcc tgctagagtg gcttcttttc tctatcactg
     8221 atagggactg cacgtcgtct ctatcactga tagggattcc gccgcttctc tatcactgat
     8281 agggacacgc ggccgtgcta gagtgatgcg ttttctctat cactgatagg gacctcccct
     8341 gctctctatc actgataggg ataccactcc ttctctatca ctgataggga tttgtttcgt
     8401 tgctagagca ctagggggtc tctatcactg atagggaccc tgctaggtct ctatcactga
     8461 tagggaagag cgtttatctc tatcactgat agggattccc ccgtctgcta gagggccctt
     8521 ccctctctat cactgatagg ggtggtctgc tctctctacc acagataggg atcaacaact
     8581 ttctctatca ctgataggga actgctactt tgctagagtg tggccctttc tctatcactg
     8641 atagggaacc atctctttct ctatcactga tagggatatt ctggtttctc tatcacttag
     8701 ggacagctac cactgctaga gtcccaggcc ctctctatca ctgataggga acgaccttac
     8761 tctctatcac tgatagggag ttcccccttt ctctatcctg atagggatcg tccatactgc
     8821 tagaggctgc cctcctctct atcactatag ggatccccct cattctctat cactgatagg
     8881 gatgtggtct cctctctatc actgataggg atctccgccg ttgctagagt gcccgcgcct
     8941 ctctatcact gatagggata tgcgtgcttc tctatcactg atagggagcc attgccttct
     9001 ctatcactga tagggattat tccttctgct agagctacta ttcttctcta tcactgatag
     9061 ggacccccgg cgctctctat cactgatagg gaggcatcgc cctctctatc actgataggg
     9121 accccctggc ttgctagaga tgcgttacct ctctatcact gatagggacc ggcccttttc
     9181 tctatcactg atagggagcc gcctctctct ctatcactga tagggatcat ttangctgct
     9241 agagtgctgc ttcctctcta ccactgatag ggacgctttt atatctctat cactgatagg
     9301 gattttggcc cttctctatc actgagaggg acgtttggcc ctgctagagc tccccgccct
     9361 ctctatcact gatagggatt cctgataggg acctgccgcg ctctctatca ctgataggga
     9421 cgttggcacc tgctagagcg ctctaccctc tctatcactg atagggaccc tgtgctttct
     9481 ctatcactga taggactcag gcctctctct atcactgata gggacatccc cccgtgctag
     9541 agactctttt tcgtctctat cactgatagg gactctccgg aatctctatc actggtaggg
     9601 acgtttgtct ctctctatca ctgataggga ctagttgcaa tgctagagtc tccccgcctc
     9661 tctatcactg atagggactt ttttccctct ctatcactga tagggatttt cgttgttctc
     9721 tatcactgat agggatacct cacctgctag agtttttaac ggtctctatc actgataggg
     9781 agtcggctcg ctctctatca ctgataggga tttatatgac tctctatcac tgatagggat
     9841 tagctcccct gctagagatt ataaggctct ctatcactga tagggatccc ccctcgtctc
     9901 tatcactaat agggatcact ccgcgtctct atcactgata gggagtcgct ctcatgctag
     9961 agtccgtctc tttctctatc actgataggg acttagcact ctctctatca ctgataggga
    10021 gcttacttcc tctctatcac tgatagggac gcctctctct gctagagtac cttccactct
    10081 ctatcactga tagggataca tcttcctccc tatcactgat agggacgtgn ataattctct
    10141 atcactatag ggatagcggc ctctgctaga gcctaccatt ctctctatca ctgataggga
    10201 ccttctccac gtctctatca ctgataggga cctcctctct tctctatcac tgatagggac
    10261 acggctccgt gctagagtcc ccgcggttct ctatcactga tagggattta aaccgctctc
    10321 tatcactgat agggatgtct cccggtctcn atcnctnata gggatcagac tccatgctag
    10381 agcctcctta cntntcnatc actgataggg atccgccngn gtctctatca ntgataggga
    10441 accgccncca tctctatcac tgatagggat ggnccccatn gctagagtta tcccttgtct
    10501 ctatcactga tngggatggc tcttcgtctc tatcactgat agggagntct gcgctctntc
    10561 tatcnactga tagggactan agaccctgct anagagtagc cccctcncta tcnctgatag
    10621 ggacacccga gattctctat cnctgaggga ttcntnagtg tctctntcnc tgatagggan
    10681 nacacttcat gntagagtgn tgatctgtct ctatcnctga tagggacatc tccccctctc
    10741 tatcnctgag ggacctctna ctttctctgt cactgatagg gacnactctt tctgctagag
    10801 tctctctcca tctctatcac tgatagggac tcggctttat ctctatcact gatagggacg
    10861 gtcccgtctc tctatcactg atagggantg ngcccnctgc tagagccagc tcgcttctct
    10921 atcactgata gggaacattt cggctctcta tcactgatag ggaggccccg cattctctat
    10981 cactgatagg gattctcggc cctgctagag atgcaccccg tctctatcac tgatagggat
    11041 agtccgcagt ctctatcact gatagggatg cgttctgttc tctatcactg tagggaccat
    11101 tgctagagtt gtcccttctc tctatcactg atagggactc cggcgtctct ctatcactga
    11161 tagggacaag cgcgcctctc tatcactgat agggaccgtc tcccctgcta gagcttcgtc
    11221 ccttctctat cactgatagg gatccatcct gttctctatc actgatagga tctataggtc
    11281 tctctatcac tgatagggac cttcccttct gctagagctg gccccattct ctatcactga
    11341 tagggaccgt ctcctttctc tatcactgat agggaccacc tgtctctcta tcactgatag
    11401 ggaccccctc gcttgctaga gcgattacct ttctctatca ctgataggga ccttccgtcg
    11461 tctctatcac tgatagggat ctcctgttct ctctatcact gatagggacc ctgcccgctg
    11521 ctagagccac acactgtctt tatcactgat agggacctcc ccacttctct atcactgata
    11581 gggacgcgca agatagggac ccgcctccgt gctagagtcc cacttcgtct ctatcactga
    11641 tagggatcct ttcccctctc tatcactgat agggacggtt cgtcttctct atcactgata
    11701 ggaccnccct gctagcctcg agggggggcc cggtacccag cttttgttcc ctttagtgag
    11761 ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    11821 cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct
    11881 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    11941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    12001 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    12061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    12121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    12181 tgctggcgtt tttccatagg ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa
    12241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgttcccc cctggaagct
    12301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    12361 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
    12421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    12481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    12541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    12601 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    12661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    12721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    12781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    12901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    13021 tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    13081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    13141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    13201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    13261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    13321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg gttagctcct
    13381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    13441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    13501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    13561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    13621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    13681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    13741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    13801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    13861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    13921 ttccccgaaa agtgccacct gacgtcttat tatcatgaca ttaacctata aaaataggcg
    13981 tatcacgagg ccctttcgtc
//
