LOCUS       sequence-64802-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGBKCg
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(321..722)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgatgc ggtacctcag
      301 gaggcttgct tcaagcttgg agttgattgt atgcttggta tagcttgaaa tattgtgcag
      361 aaaaagaaac aaggaagaaa gggaacgaga acaatgacga ggaaacaaaa gattaataat
      421 tgcaggtcta tttatacttg atagcaagac agcaaacttt tttttatttc aaattcaagt
      481 aactggaagg aaggccgtat accgttgctc attagagagt agtgtgcgtg aatgaaggaa
      541 ggaaaaagtt tcgtgtgctt cgagataccc ctcatcagct ctggaacaac gacatctgtt
      601 ggtgctgtct ttgtcgttaa ttttttcctt tagtgtcttc catcattttt ttgtcattgc
      661 ggatatggtg agacaacaac gggggagaga gaaaagaaaa aaaaagaaaa gaagttgcat
      721 gcgcctatta ttacttcaat agatggcaaa tggaaaaagg gtagtgaaac ttcgatatga
      781 tgatggctat caagtctagg gctacagtat tagttcgtta tgtaccacca tcaatgaggc
      841 agtgtaattg gtgtagtctt gtttagccca ttatgtcttg tctggtatct gttctattgt
      901 atatctcccc tccgccacct acatgttagg gagaccaacg aaggtattat aggaatcccg
      961 atgtatgggt ttggttgcca ga
//
