LOCUS       sequence-64803-e        8048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Finney-Manchester SP, Maheshri N
  TITLE     Harnessing mutagenic homologous recombination for targeted 
            mutagenesis in vivo by TaGTEAM.
  JOURNAL   Nucleic Acids Res. 2013 Mar 7.
  PUBMED    23470991
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Mar 7."
COMMENT     Sequence Label: pBS-85xtetO-KlURA3
FEATURES             Location/Qualifiers
     source          1..8048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2063..2081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2092..2110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2139..2157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2167..2185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2196..2214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2243..2261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2300..2318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2347..2365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2404..2422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2451..2469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2480..2498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2686..2704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2782..2800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2811..2829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2858..2876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2887..2905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2916..2934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2963..2981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2992..3010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3021..3039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3171..3189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3200..3218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3275..3293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3304..3322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3333..3351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3379..3397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3408..3426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3502..3520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3531..3549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3578..3596
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3607..3625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3636..3654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3683..3701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3712..3730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3741..3759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3788..3806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3817..3835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3846..3864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3921..3939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3996..4014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4025..4043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4054..4072
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4101..4119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4130..4148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4159..4177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4233..4251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4262..4280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4309..4327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4338..4356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4367..4385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4414..4432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4443..4461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4472..4490
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4519..4537
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4548..4566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4577..4595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4624..4642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4680..4698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4727..4745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4756..4774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4785..4803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4832..4850
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4861..4879
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4890..4908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4937..4955
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4966..4984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5038..5056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5067..5085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5096..5114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5143..5161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5229..5245)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5245)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5860..5880)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5860..5878)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5899..5915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5899..5915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5912..5934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5923..5939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5947..5953,5954..5971,5972..5977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5992..6013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6130..6147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6301..6889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6381..6400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7060..7851,7852..7920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7683..7702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7921..8025)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccggagac aatcatatgg gagaagcaat tggaagatag aaaaaaggta ctcggtacat
      721 aaatatatgt gattctgggt agaagatcgg tctgcattgg atggtggtaa cgcatttttt
      781 tacacacatt acttgcctcg agcatcaaat ggtggttatt cgtggatcta tatcacgtga
      841 tttgcttaag aattgtcgtt catggtgaca cttttagctt tgacatgatt aagctcatct
      901 caattgatgt tatctaaagt catttcaact atctaagatg tggttgtgat tgggccattt
      961 tgtgaaagcc agtacgccag cgtcaataca ctcccgtcaa ttagttgcac catgtccaca
     1021 aaatcatata ccagtagagc tgagactcat gcaagtccgg ttgcatcgaa acttttacgt
     1081 ttaatggatg aaaagaagac caatttgtgt gcttctcttg acgttcgttc gactgatgag
     1141 ctattgaaac tagttgaaac gttgggtcca tacatttgcc ttttgaaaac acacgttgat
     1201 atcttggatg atttcagtta tgagggtact gtcgttccat tgaaagcatt ggcagagaaa
     1261 tacaagttct tgatatttga ggacagaaaa ttcgccgata tcggtaacac agtcaaatta
     1321 caatatacat cgggcgttta ccgtatcgca gaatggtctg atatcaccaa cgcccacggg
     1381 gttactggtg ctggtattgt tgctggcttg aaacaaggtg cgcaagaggt caccaaagaa
     1441 ccaaggggat tattgatgct tgctgaattg tcttccaagg gttctctagc acacggtgaa
     1501 tatactaagg gtaccgttga tattgcaaag agtgataaag atttcgttat tgggttcatt
     1561 gctcagaacg atatgggagg aagagaagaa gggtttgatt ggctaatcat gaccccaggt
     1621 gtaggtttag acgacaaagg cgatgcattg ggtcagcagt acagaaccgt cgacgaagtt
     1681 gtaagtggtg gatcagatat catcattgtt ggcagaggac ttttcgccaa gggtagagat
     1741 cctaaggttg aaggtgaaag atacagaaat gctggatggg aagcgtacca aaagagaatc
     1801 agcgctcccc attaattata caggaaactt aatagaacaa atcacatatt taatctaata
     1861 gccacctgca ttggcacggt gcaacactac ttcaacttca tcttacaaaa agatcacgtg
     1921 atctgttgta ttgaactgaa aattttttgt ttgcttctct ctctctcttt cattatgtga
     1981 gatttaaaaa ccagaaacta catcatcgaa aaagagtttt aaaccattac aaccattgcg
     2041 ataagcaagc ttgatatcga attccctatc agtgatagag aaatatggca atccctatca
     2101 gtgatagaga tcatagcggc ctctagcaga gttgggggtc cctatcagtg atagagagta
     2161 tgtagttccc tatcagtgat agagatagag aggaatccct atcagtgata gagagggaga
     2221 agagctctag caagggggta cgtccctatc agtgatagag atagggggag gccctatcag
     2281 tgatagagag cgaggggaat ccctatcagt gatagagaag gggactcact ctagcaacga
     2341 tacaggtccc tatcagtgat agagaggata gccagtccct atcagtatag agaactgggc
     2401 aaatccctat cagtgataga gaaaatggca tgctctagca gaaaatcaag tccctatcag
     2461 tgatagagag gtgaggagct ccctatcagt gatagagagg gttatcagtc cctatcagtg
     2521 atagagaaaa atagagtctc tagcagggaa gggtccctat ccgtgataga gaagaataag
     2581 tatccctatc agtgatagag ccaaagcatg atccctatag tgatagagat ggattcaact
     2641 ctagcaccgg aaaagttccc tatcagtggt agagaagctg aaaagtccct atcagtgata
     2701 gagaggcgaa tgactcccta tcagtgagga gcggctctag caaagagagg agtccctatc
     2761 agtgatagag atcagaggca ctccctatca gtgatagaga gggggataca tccctatcag
     2821 tgatagagaa agcgttggac tctagcagcg aagcgattcc ctatcagtga tagagaaagg
     2881 agaggatccc tatcagtgat agagagtggg gcgcatccct atcagtgata gagacgaggt
     2941 ccagctctag caagcaggtc catccctatc agtgatagag agaaggggag gtccctatca
     3001 gtgatagaga aaaggatgga tccctatcag tgatagagag cgatcggaac tctagcagac
     3061 taagagttcc tatcagtgat agagacaaga aagcatncct ancagtgnta gagagagcgc
     3121 aagtccctat cagtgataga gagacagggc agctctagca cgcgtaagga tccctatcag
     3181 tgatagagat cggtgaatat ccctatcagt gatagagacg ggagggcctc ctatcagtga
     3241 tagagataca tagaggctct agcaaaagcg aacctcccta tcagtgatag agaggtgcgt
     3301 gaatccctat cagtgataga gaaagccagc gttccctatc agtgatagag aggcaaacat
     3361 gctctagcag aggtttactc cctatcagtg atagagaaga aaggacttcc ctatcagtga
     3421 tagagagaaa agcgactcag tgatagagaa ggacgaaatc tctagcagtc aatgggctcc
     3481 cttgatagag aaaatacggg ttccctatca gtgatagaga ggagaggggc tccctatcag
     3541 tgatagagag cacgcaggac tctagcacgt aaagtgctcc ctatcagtga tagagaagcg
     3601 ggaaaatccc tatcagtgat agagagctac agagctccct atcagtgata gagatctcag
     3661 agggctctag caaacgggag agtccctatc agtgatagag agagttaaga ctccctatca
     3721 gtgatagaga cgcatcacgg tccctatcag tgatagagac acgtttgaac tctagcagaa
     3781 gtgcggatcc ctatcagtga tagagactaa agaatttccc tatcagtgat agagaacatt
     3841 cacgttccct atcagtgata gagagtgtgg gcgcctctag catcgagggg aatccctatg
     3901 agtgatagag acgagataag tccctatcag tgatagagac gatatgagtc cctatcagtg
     3961 gtagagaggg gacatcgctc tagcagtggg atggatccct atcagtgata gagaacacag
     4021 gccatcccta tcagtgatag agaggcgtaa cactccctat cagtgataga gatacagaca
     4081 atctctagca agcatgaggc tccctatcag tgatagagag cggacgggct ccctatcagt
     4141 gatagagagt aggggccatc cctatcagtg atagagagaa aaaggaactc tagcactggg
     4201 agggtcctta tcagtgatag agagaatata ggtccctatc agtgatagag agccgtcacg
     4261 ctccctatca gtgatagaga gagggggtgc ctctagcact ccgcgggctc cctatcagtg
     4321 atagagaggc aaggaagtcc ctatcagtga tagagaaatc gatcgttccc tatcagtgat
     4381 agagatgggt ctaaactcta gcatagcaga agctccctat cagtgataga gaggggggag
     4441 attccctatc agtgatagag agacaggaaa atccctatca gtgatagaga cgctncagca
     4501 ctctagcaca aaccctggtc cctatcagtg atagagaggg atcgtaatcc ctatcagtga
     4561 tagagaagag catggatccc tatcagtgat agagagcgcg cagggctcta gcacgtcatg
     4621 gtatccctat cagtgataga gagcacgcag accctatcag tgatagagag aggcaggggt
     4681 ccctatcagt gatagagaca ggtcccgact ctagcatcgt gacaagtccc tatcagtgat
     4741 agagaacccc gagcctccct atcagtgata gagagaatca gaggtcccta tcagtgatag
     4801 agaaacacgg gtcctctagc aagaagatca atccctatca gtgatagaga aaaggtgaga
     4861 tccctatcag tgatagagac tcagcgctgt ccctatcagt gatagagaat gcagggagct
     4921 ctagcagaat gacaggtccc tatcagtgat agagacccaa ggtcgtccct atcagtgata
     4981 gagagctgtg catctcccta tcagtgatag accgcaagaa gctctagcag agcagggtcc
     5041 ctatcagtga tagagaagcg gaaccatccc tatcagtgat agagaagaat agaagtccct
     5101 atcagtgata gagagatata ccaactctag caaaggccgg gatccctatc agtgatagag
     5161 acagcacgga gaatgcagcg cctccctatc agtgaaagga gaagaagtgg ctctagcaag
     5221 gcccggggga tccactagtt ctagaatttt gacatatact gatatggacc tcttgtttcg
     5281 tataaatcgc tatttatttc cccagataac taaagaaaat ccttcaaccc agcgttctat
     5341 attactatat tctcaacccg ccgttttcct gctgtgcgat aattctcatt caatactacc
     5401 atttcaaacc tagaaaaggg tgtctttatt aaaactgtaa gaaaatttta tcatgaactc
     5461 tgatgaaaag tcttcggaag atcaactttt tgatttttca aaaatgaaag aaactgttgt
     5521 ctccatttat ggcctcatat ctgaaggcgc gcccaatcgc tgggaatgag caataccatc
     5581 tgttttttac tgcaaagtac agatttatat gcttcttggc caccgtgaat cagtaagcaa
     5641 agaatttttg aaagcattag gttggccggt ttgtaatctc atggaaacca tgttgtatgt
     5701 tactactgat tttcaatcta tgtagtttta ccgaagctaa tgagttttga taatcactaa
     5761 caatcaattg cttgccgacg tctccatgat cttagtttga gtatctttgt gaactacttg
     5821 aaagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     5881 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6721 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8041 agtgccac
//
