LOCUS       sequence-64804-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-GFP-IKBalpha-mut (super repressor)
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(62..78)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(63..79)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             84..803
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
ORIGIN
        1 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
       61 tcgataccgt cgacctcgag accatggtga gcaagcagat cctgaagaac accggcctgc
      121 aggagatcat gagcttcaag gtgaacctgg agggcgtggt gaacaaccac gtgttcacca
      181 tggagggctg cggcaagggc aacatcctgt tcggcaacca gctggtgcag atccgcgtga
      241 ccaagggcgc ccccctgccc ttcgccttcg acatcctgag ccccgccttc cagtacggca
      301 accgcacctt caccaagtac cccgaggaca tcagcgactt cttcatccag agcttccccg
      361 ccggcttcgt gtacgagcgc accctgcgct acgaggacgg cggcctggtg gagatccgca
      421 gcgacatcaa cctgatcgag gagatgttcg tgtaccgcgt ggagtacaag ggccgcaact
      481 tccccaacga cggccccgtg atgaagaaga ccatcaccgg cctgcagccc agcttcgagg
      541 tggtgtacat gaacgacggc gtgctggtgg gccaggtgat cctggtgtac cgcctgaaca
      601 gcggcaagtt ctacagctgc cacatgcgca ccctgatgaa gagcaagggc gtggtgaagg
      661 acttccccga gtaccacttc atccagcacc gcctggagaa gacctacgtg gaggacggcg
      721 gcttcgtgga gcagcacgag accgccatcg cccagctgac cagcctgggc aagcccctgg
      781 gcagcctgca cgagtgggtg taatagggta ccttcgataa aataaaagat tttatttagt
      841 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
      901 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
      961 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     1021 tgccccggct cagggccaag aacag
//
