LOCUS       sequence-64813-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Hammer BK, Swanson MS
  TITLE     Co-ordination of legionella pneumophila virulence with entry into 
            stationary phase by ppGpp.
  JOURNAL   Mol Microbiol. 1999 Aug;33(4):721-31.
  PUBMED    10447882
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol."; date: "1999-08"; volume: "33(4)"; pages: "721-31"
COMMENT     Sequence Label: pTLPflaG-small
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(231..247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(231..247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(244..266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(279..285,286..303,304..309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    324..345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(462..479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(713..732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgaaaagtt cttctccttt actcatatgt atatctcctt cttaaatcta gacctgaatc
       61 cctggtatgg gacatttgta attcagtaaa ttcgatttat tgttgtgaat ttactgtacc
      121 cgatttagtt atcggaaata gtgaaaaaaa ctttagttcc gaaagcgctt ttttgaaaag
      181 tcgcaaagga tccgcggccg cccgggcccg gtaccgaatt cgtaatcatg gtcatagctg
      241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      781 ggatacctgt ccgcctttct c
//
