LOCUS       sequence-64831-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: pHIS2-KL EAT
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             603..614
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             721..729
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gattccaaca taactcctgc agtggaatcg cttgcggcca aagcatttgt catgtattcg
       61 ttcgctgaaa tgaaattctc tcaaattctt ccaactcctc cttcttcaac cgactatgac
      121 ccactgtcag ataaacgtct aagtaacgga agttgcgcga tagaggatga agaagatttg
      181 gatcaaggcc gcccaccttc taatcaaact ttgacctcag caacaacaaa gatttcatca
      241 gccaccaatg ttgacactca aataccggcc cccgagttga aaaagttgtg caccgaatct
      301 ttacttcttt atctaaaagc tcttactatt ttagctgctt caatgaaatt aacatcaaaa
      361 tggtggtatg aaaatgaaag taaaaactgc actttgaagc taaacatttt ggtgcagtgg
      421 attagagatc gctttaacga atgcttagac aaggctgaat ttttgcgttt aaagctccat
      481 gctattaata catcaccaaa ttcccaatgg tcagatgatg accctgtaat ttttgttgaa
      541 aagttaatat acgatcgtgc tttagatata tctagaaatg ctgcaagaat ggaaatggaa
      601 agtgggaatt acaatacttg tgaactagcc tatgctactt cattatggat gttagaaatt
      661 ttgctggacg aaaattttca attcaacgaa gtctatgatg acgagtatgc atcaaatatc
      721 acctccttag acgaatcaga taaggaaatg ataaagaaat acatttcaag tattgctaat
      781 aggttaaagg cactaaaatc caaaatggtt tag
//
