LOCUS       sequence-64836-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL018A
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             765..787
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagttat ttatacagct
       61 catccatacc accggtagaa tgacgtcctt cagcacgctc atactgctcc acgatcgtat
      121 agtcctcatt gtgtgaggtg atgtccagtt tgatgttaac gttataggca ccaggcagtt
      181 ggacaggttt tttggcttta tacgtggttt tcacttcggc atcatagtgg ccaccatctt
      241 tcagtttcag acgctgtttg atttcgcctt tcagagcgcc atcttctgga tacatacgct
      301 cactagaggc ctcccaaccc atcgtttttt tctgcataac cgggccatca ctcggaaaat
      361 tcgtgccacg cagtttcact ttatagatga actcaccgtc ttgcaggcta gaatcctgtg
      421 tcactgtcac aacaccacca tcctcaaagt tcatcacacg ttcccatttg aacccttcag
      481 ggaagctcag tttcagatag tccggaatat cggccggatg tttcacatag gctttggagc
      541 catacatgaa ctgtgggctc agaatatccc aagcaaatgg cagtggacca cctttcgtga
      601 ctttcagttt agcggtctgt gtaccctcat acggacgacc ttcgccttcg ccctcaattt
      661 cgaactcgtg accattaacg ctgccttcca tgtggacttt gaagcgcatg aactctttga
      721 tgatcgccat attgtcctcc tcgcctttac tcaccatatg tatatctcct tcttaaagtt
      781 aaacaaaatt attcggatcc gcttatttat acagttcatc cataccca
//
