LOCUS       sequence-64837-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL018A
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             773..795
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg gtgagtaaag gcgaagagct
       61 gttcacaggg gttgttccga ttctggtcga actggacggg gacgttaatg gtcacaaatt
      121 cagcgttagc ggtgagggcg agggtgatgc cacttatggt aaactgaccc tgaaattcat
      181 ctgtaccacc ggcaaactgc ctgttccttg gcctacactg gttacaacac tgacttgggg
      241 tgttcaatgt tttgctcgct atccggatca catgaaacag cacgatttct tcaaaagcgc
      301 catgcctgaa ggttatgtcc aagagcgtac gatcttcttt aaagacgacg gcaactataa
      361 aacccgtgcc gaggtgaaat tcgaaggtga taccctggta aaccgtatcg aactgaaagg
      421 gatcgacttc aaagaggacg ggaacattct gggccataaa ctggagtata acgccatcag
      481 cgataatgtg tatattaccg ccgacaaaca gaaaaacggg atcaaagcca acttcaaaat
      541 ccgccacaac atcgaggatg gtagcgttca actggccgat cactatcaac agaatacccc
      601 gattggtgat ggtcctgttc tgctgcctga taaccactat ctgagcaccc agtctaaact
      661 gtccaaagac ccgaacgaga aacgtgatca catggttctg ctggagtttg ttaccgctgc
      721 cggcattact ctgggtatgg atgaactgta taaataagcg gatccgaata attttgttta
      781 actttaagaa ggagatatac atatggtgag taaaggcgag gaggacaata tggcgatcat
      841 caaagagttc atgc
//
