LOCUS       sequence-64854-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A>
            HP1aCS...
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..48
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             join(55..228,229..234,235..417)
                     /codon_start=1
                     /product="composite human DNA-binding domain composed of 
                     domains from the transcription factors Zif268 and Oct-1 
                     (Pomerantz et al., 1995)"
                     /label=ZFHD1
                     /label=ZFHD1 (DNA binding domain)
                     /translation="ERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSR
                     SDHLTTHIRTHTGGGRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKE
                     VIRVWFCNRRQKEKRIN"
     protein_bind    complement(431..455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             487..807
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             487..807
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             814..840
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggccgcgcca ccatggacta tcctgctgcc aagagggtca agttggactc tagagaacgc
       61 ccatatgctt gccctgtcga gtcctgcgat cgccgctttt ctcgctcgga tgagcttacc
      121 cgccatatcc gcatccacac aggccagaag cccttccagt gtcgaatctg catgcgtaac
      181 ttcagtcgta gtgaccacct taccacccac atccgcaccc acacaggcgg cggccgcagg
      241 aggaagaaac gcaccagcat agagaccaac atccgtgtgg ccttagagaa gagtttcttg
      301 gagaatcaaa agcctacctc ggaagagatc actatgattg ctgatcagct caatatggaa
      361 aaagaggtga ttcgtgtttg gttctgtaac cgccgccaga aagaaaaaag aatcaacaag
      421 ggcgaattcg acccagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa
      481 ctcgagggcg tgcaggtgga gactatctcc ccaggagacg ggcgcacctt ccccaagcgc
      541 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa atttgattcc
      601 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
      661 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
      721 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
      781 ttcgatgtgg agcttctaaa actggaagtc gactatccgt acgacgtacc agactacgca
      841 accggtgaag gtcgtggatc tcttctcact tgtggcgatg ttgaaa
//
