LOCUS       sequence-64855-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N163 nLV
            EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-...
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(391..415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             432..698
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 ggatccgcgg ccgcgccacc atgaaggagg gtgaaaacaa taagcccagg gagaaatcag
       61 aaggaaacaa gaggaaatcc agtttctcca acagcgctga tgatattaaa tctaaaaaaa
      121 agagagagca aagcaatgat atcgctcggg gctttgagag aggactggaa ccagaaaaga
      181 tcatcggagc aacagattcc tgcggtgact taatgttctt aatgaaatgg aaagacacag
      241 atgaagctga cctggttctt gcaaaagaag ctaacgtgaa gtgtccacag attgtgatag
      301 cattttatga agagagactg acgtggcacg catatccaga ggatgcggaa aacaaagaaa
      361 aagaaagcgc gaagagcaag ggcgaattcg acccagcttt cttgtacaaa gtggttgatc
      421 tagagggccc tatgtggcat gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa
      481 ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg gaacggggcc
      541 cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta atggaggccc
      601 aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcacc caagcctggg
      661 acctctatta tcatgtgttc cgacgaatct caaagcagtt cggcggcgga ggtagcggcg
      721 gtggatcaat gtggcacgag ggattggagg aagctagcag actttatttc ggtgagcgca
      781 atgtcaaggg aatgttcgag gtcctcgaac ctctccacgc catgatggag agaggacctc
      841 aaaccctcaa agagact
//
