LOCUS       sequence-64859-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N114 nLV EF-1a-Gal4-VP16-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..323)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             complement(324..344)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(351..791)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attcacgcgt
       61 tcgcgatcta gagccggcgc ggccgcctac ccaccgtact cgtcaattcc aagggcatcg
      121 gtaaacatct gctcaaactc gaagtcggcc atatccagag cgccgtaggg ggcggagtcg
      181 tggggggtaa atcccggacc cggggaatcc ccgtccccca acatgtccag atcgaaatcg
      241 tctagcgcgt cggcatgcgc catcgccacg tcctcgccgt ctaagtggag ctcgtccccc
      301 aggctgacat cggtcggggg ggcgacttta cgcttctttt tagggcccat cgatacagtc
      361 aactgtcttt gacctttgtt actactctct tccgatgatg atgtcgcact tattctatgc
      421 tgtctcaatg ttagaggcat atcagtctcc actgaagcca atctatctgt gacggcatct
      481 ttattcacat tatcttgtac aaataatcct gttaacaatg cttttatatc ctgtaaagaa
      541 tccattttca aaatcatgtc aaggtcttct cgaggaaaaa tcagtagaaa tagctgttcc
      601 agtctttcta gccttgattc cacttctgtc agatgtgccc tagtcagcgg agaccttttg
      661 gttttgggag agtagcgaca ctcccagttg ttcttcagac acttggcgca cttcggtttt
      721 tctttggagc acttgagctt tttaagtcgg caaatatcgc atgcttgttc gatagaagac
      781 agtagcttca tggtggcgcg gccgcggatc ctcacgacac ctgaaatgga agaaaaa
//
