LOCUS       sequence-64860-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A>
            HP1aCS...
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..139)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(146..412)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             complement(443..709)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     protein_bind    726..750
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttcacgcgtt
       61 cgcgatctag agccggcgcg gccgcctaac cggtacgcgt agaatcgaga ccgaggagag
      121 ggttagggat aggcttacct tcgaattgtt tgctaatcct cctaaagacg tggtagtaca
      181 aatcccaagc ctgtgtcaaa tctttcacgt tgccgctctt catatatttg cgacaccatt
      241 cctgagcttc catcaggtcc ctgccgtaag cttggttgaa actagtctct ttgagggttt
      301 gaggtcctct ctccatcatg gcgtggagag gttcgaggac ctcgaacatt cccttgacat
      361 tgcgctcacc gaaataaagt ctgctagctt cctccaatcc ctcgtgccac attgatccac
      421 cgccgctacc tccgccgccg aactgctttg agattcgtcg gaacacatga taatagaggt
      481 cccaggcttg ggtgaggtcc ttgacattcc ctgatttcat gtacttcctg caccactctt
      541 gggcctccat taaatctcga ccataggcct gattaaagga tgtttccttc agagtctggg
      601 ggccccgttc catcatagca tgcaagggct ccagcacctc aaacatgcct ttcacgttcc
      661 tttccccaaa gtacaaacga gatgcctctt ccaggccttc atgccacata gggccctcta
      721 gatcaaccac tttgtacaag aaagctgggt cgaaat
//
