LOCUS       sequence-64861-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N113 nLV EF-1a-Gal4-Stop-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..530)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          560..763
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(583..603)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attcacgcgt
       61 tcgcgatcta gagccggcgc ggccgcctac gatacagtca actgtctttg acctttgtta
      121 ctactctctt ccgatgatga tgtcgcactt attctatgct gtctcaatgt tagaggcata
      181 tcagtctcca ctgaagccaa tctatctgtg acggcatctt tattcacatt atcttgtaca
      241 aataatcctg ttaacaatgc ttttatatcc tgtaaagaat ccattttcaa aatcatgtca
      301 aggtcttctc gaggaaaaat cagtagaaat agctgttcca gtctttctag ccttgattcc
      361 acttctgtca gatgtgccct agtcagcgga gaccttttgg ttttgggaga gtagcgacac
      421 tcccagttgt tcttcagaca cttggcgcac ttcggttttt ctttggagca cttgagcttt
      481 ttaagtcggc aaatatcgca tgcttgttcg atagaagaca gtagcttcat ggtggcgcgg
      541 ccgcggatcc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct
      601 tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt
      661 gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa
      721 acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc
      781 tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc
      841 ttttcc
//
