LOCUS       sequence-64862-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N163 nLV
            EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-...
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..140)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(147..413)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             complement(444..710)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     protein_bind    727..751
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attcacgcgt
       61 tcgcgatcta gagccggcgc ggccgcctaa ccggtacgcg tagaatcgag accgaggaga
      121 gggttaggga taggcttacc ttcgaattgt ttgctaatcc tcctaaagac gtggtagtac
      181 aaatcccaag cctgtgtcaa atctttcacg ttgccgctct tcatatattt gcgacaccat
      241 tcctgagctt ccatcaggtc cctgccgtaa gcttggttga aactagtctc tttgagggtt
      301 tgaggtcctc tctccatcat ggcgtggaga ggttcgagga cctcgaacat tcccttgaca
      361 ttgcgctcac cgaaataaag tctgctagct tcctccaatc cctcgtgcca cattgatcca
      421 ccgccgctac ctccgccgcc gaactgcttt gagattcgtc ggaacacatg ataatagagg
      481 tcccaggctt gggtgaggtc cttgacattc cctgatttca tgtacttcct gcaccactct
      541 tgggcctcca ttaaatctcg accataggcc tgattaaagg atgtttcctt cagagtctgg
      601 gggccccgtt ccatcatagc atgcaagggc tccagcacct caaacatgcc tttcacgttc
      661 ctttccccaa agtacaaacg agatgcctct tccaggcctt catgccacat agggccctct
      721 agatcaacca ctttgtacaa gaaagctggg tcgaattcgc ccttgctctt cgcgctttct
      781 ttttctttgt tttccgcatc ctctggatat gcgtgccacg tcagtctctc ttcataaaat
      841 gctatcacaa tctgtggaca cttcacgtta gcttcttttg caagaacc
//
