LOCUS       sequence-64863-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression plasmid of human codon-optimized SpCas9 and a gRNA 
            to the human Emx1 locus, can be used to test SpCas9 cleavage in cell
            lines of choice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: 
            pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..61)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctaggaattc ttactttttc ttttttgcct ggccggcctt tttcgtggcc gccggccttt
       61 tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg atgctctggt
      121 ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg tcgatggtgg
      181 tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac aggtggatga
      241 tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg gacagcactt
      301 tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc tgctcgatga
      361 tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca ttatcctcgg
      421 gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca tatttggagg
      481 gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt ctcttccggc
      541 cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc tttttcactt
      601 ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg cttctttcca
      661 tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg cccttttcca
      721 ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg ccgtacttct
      781 tagggtccca gtccttcttt ctggcgatca gcttatcgct gttcctcttg gg
//
