LOCUS       sequence-64865-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..122)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(129..449)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(129..449)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    481..505
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(511..522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attcacgcgt
       61 tcgcgatcta gagccggcgc ggccgcctaa ccggttgcgt agtctggtac gtcgtacgga
      121 tagtcgactt ccagttttag aagctccaca tcgaagacga gagtggcatg tggtgggatg
      181 atgcctgggt gcccagtggc accataggca taatctggag atatagtcag tttggctctc
      241 tgacccacac tcatctgggc aaccccttct tcccagcctc ggatcacctc ctgcttgcct
      301 agcataaact taaagggctt gtttctgtcc cgggaggaat caaatttctt tccatcttca
      361 agcatcccgg tgtagtgcac cacgcaggtc tggccgcgct tggggaaggt gcgcccgtct
      421 cctggggaga tagtctccac ctgcacgccc tcgagttcga accgcgggcc ctctagatca
      481 accactttgt acaagaaagc tgggtcgaat tcgcccttcg atacagtcaa ctgtctttga
      541 cctttgttac tactctcttc cgatgatgat gtcgcactta ttctatgctg tctcaatgtt
      601 agaggcatat cagtctccac tgaagccaat ctatctgtga cggcatcttt attcacatta
      661 tcttgtacaa ataatcctgt taacaatgct tttatatcct gtaaagaatc cattttcaaa
      721 atcatgtcaa ggtcttctcg aggaaaaatc agtagaaata gctgttccag tctttctagc
      781 cttgattcca cttctgtcag atgtgcccta gtcagcggag accttttggt tttgggagag
      841 tagcgacact cccagttgtt cttcagacac ttggcgcact tcggttttt
//
