LOCUS       sequence-64867-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pIRESN2 GFP-uvr8at
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      167..176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(212..240)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     835..855
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tcgatatctg cggcctagct agcgctaccg gactcagatc
      121 tcgagctcaa gcttcgaatt ctaagtgagt aaaggaccca ccggtcgcca ccatggaatc
      181 ctctagcaaa ggagaagaac tcttcactgg agttgtccca attcttgttg aattagatgg
      241 tgatgttaac ggccacaagt tctctgtcag tggagagggt gaaggtgatg caacatacgg
      301 aaaacttacc ctgaagttca tctgcactac tggcaaactg cctgttcctt ggccaacact
      361 agtcactact ctgtgctatg gtgttcaatg cttttcaaga tacccggatc atatgaaacg
      421 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgta ccatctactt
      481 caaagatgac ggcaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      541 taatagaatc gagttaaaag gtattgactt caaggaagat ggcaacattc tgggacacaa
      601 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      661 gatcaaagct aacttcaaga cccgccacaa cattgaagat ggaagcgttc aactagcaga
      721 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      781 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      841 tcttgagttt gtaacagctg ctg
//
