LOCUS       sequence-64869-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pCMV-BD-cop1at(335-675)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..50
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             87..527
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tcagatccgc tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
       61 tgggatctaa gctagcctcc tgaaagatga agctactgtc ttctatcgaa caagcatgcg
      121 atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc
      181 tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg ctgactaggg
      241 cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt ctactgattt
      301 ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat ataaaagcat
      361 tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca gatagattgg
      421 cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt gcgacatcat
      481 catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg gaattgggat
      541 ccgggtatag caacggcctt gcagattttc aatctgtgtt gactaccttc actcgctaca
      601 gtcgtctaag agttatagca gaaatccggc atggggatat atttcattca gccaacattg
      661 tatcaagcat agagtttgat cgtgatgatg agctgtttgc cactgctggt gtttctagat
      721 gtataaaggt ttttgacttc tcttcggttg taaatgaacc agcagatatg cagtgtccga
      781 ttgtggagat gtcaactcgg
//
