LOCUS       sequence-64871-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Tschaharganeh DF, Chen X, Latzko P, Malz M, Gaida MM, Felix K, Ladu 
            S, Singer S, Pinna F, Gretz N, Sticht C, Tomasi ML, Delogu S, Evert 
            M, Fan B, Ribback S, Jiang L, Brozzetti S, Bergmann F, Dombrowski F,
            Schirmacher P, Calvisi DF, Breuhahn K
  TITLE     Yes-Associated Protein Up-regulates Jagged-1 and Activates the NOTCH
            Pathway in Human Hepatocellular Carcinoma.
  JOURNAL   Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 
            10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.
  PUBMED    23419361
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.02.009"; journalName: "Gastroenterology"; 
            date: "2013-06"; volume: "144"; issue: "7"; pages: "1530-1542.e12"
COMMENT     Sequence Label: pT3-EF1a-YapS127A
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             118..141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnagnagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
       61 caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac caccatggac
      121 tacaaagacg atgacgataa agcaaggctc gaatcggtac ctatggatcc cgggcagcag
      181 ccgccgcctc aaccggcccc ccagggccaa gggcagccgc cttcgcagcc cccgcagggg
      241 cagggcccgc cgtccggacc cgggcaaccg gcacccgcgg cgacccaggc ggcgccgcag
      301 gcaccccccg ccgggcatca gatcgtgcac gtccgcgggg actcggagac cgacctggag
      361 gcgctcttca acgccgtcat gaaccccaag acggccaacg tgccccagac cgtgcccatg
      421 aggctccgga agctgcccga ctccttcttc aagccgccgg agcccaaatc ccactcccga
      481 caggccagta ctgatgcagg cactgcagga gccctgactc cacagcatgt tcgagctcat
      541 gcctctccag cttctctgca gttgggagct gtttctcctg ggacactgac ccccactgga
      601 gtagtctctg gcccagcagc tacacccaca gctcagcatc ttcgacagtc ttcttttgag
      661 atacctgatg atgtacctct gccagcaggt tgggagatgg caaagacatc ttctggtcag
      721 agatacttct taaatcacat cgatcagaca acaacatggc aggaccccag gaaggccatg
      781 ctgtcccaga tgaacgtcac agcccccacc agtccaccag tgcagcagaa tatgatgaac
      841 tcggcttcag gtcctcttcc tgatggatgg gaacaagcca tgactcnnnt ggagaaattt
      901 actatataaa ccataagaca gaccacctct tggctagacc caagcttgac ccncgttttg
      961 catgacagag natcagtcag anngctcnnn naaaca
//
