LOCUS       sequence-64886-e        3872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Metzger T, Gache V, Xu M, Cadot B, Folker ES, Richardson BE, Gomes 
            ER, Baylies MK
  TITLE     MAP and kinesin-dependent nuclear positioning is required for 
            skeletal muscle function.
  JOURNAL   Nature. 2012 Mar 18;484(7392):120-4. doi: 10.1038/nature10914.
  PUBMED    22425998
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-18- 18"; volume: "484"; issue: "7392"; 
            pages: "120-4"
COMMENT     Sequence Label: pGEMT-Map7-1333-end
FEATURES             Location/Qualifiers
     source          1..3872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(997..1015)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(998..1015)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1033..1049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1033..1049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1046..1068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1057..1073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1081..1087,1088..1105,1106..1111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1126..1147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1264..1281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1435..2023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1515..1534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2194..2985,2986..3054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2817..2836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3055..3159)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3237..3692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3374..3395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3586..3605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3818..3840
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3832..3849
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3833..3849
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3856..3872,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3856..3872,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tatgggcggt gtagttccta agacttctgc aggcaccacc gacccagagg aggctacgag
      121 gttgctggct gagaagaggc gtctagccag agagcagagg gagaaggagg aacgggagag
      181 gaaggaaaag gaagagctgg agagacaaaa gatagaggaa ttggcccgta gggtggctga
      241 agagcgaagt cgcagggaag aagaagcccg caggctggaa gaggagcagg ctcgagagaa
      301 agaggagctg gcgctgcgtc tggctgagga ggagcgggag cggtgggaga gggaggaggt
      361 ggagcgcgtg cagaagcaga aagaagaaga agcccgagcc cgggaggagg cagagagggc
      421 tcggcaggaa cgagagaagc atttccagaa agaagagcag gaacggctgg agaggaagaa
      481 gcgacttgaa gagattatga gaagaaccag gaggacagag accgctgata agaaaaccac
      541 tgagcaaaga aatggtgaca tagccaaagg agttctcact ggagagccag aagtacctgc
      601 actgccgtgt atggcctctt caggaaacgg agagtctgca gagagcccac atggagtcgc
      661 tttacagcaa tcagaagtga ccacagagag ttctccagat ttggaaaaac agccaaatga
      721 aaacggaatg tccatacaaa atgaaaattt tgaagaagtt ataaacttac ctgttggatc
      781 aaaagcgtcc agattagatg tcaccaatga gaacccagaa attcctttga aaccaatttt
      841 ggcctttaat gatgaaggga cacttgggcc cctacctcag gtggatggtg tgcagacaca
      901 acagaccgca gaagttaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg
      961 agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt
     1021 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3181 acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     3241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3661 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3721 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3781 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3841 acggccagtg aattgtaata cgactcacta ta
//
