LOCUS       sequence-64898-e       11731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Swanson C, Saintigny Y, Emond MJ, Monnat RJ Jr
  TITLE     The Werner syndrome protein has separable recombination and survival
            functions.
  JOURNAL   DNA Repair (Amst). 2004 May 4;3(5):475-82.
  PUBMED    15084309
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Repair 
            (Amst)."; date: "2004-05-4"; volume: "3(5)"; pages: "475-82"
COMMENT     Sequence Label: pMM288
FEATURES             Location/Qualifiers
     source          1..11731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5003..5032)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5042..5071)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5081..5110)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5120..5149)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5159..5188)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    join(5343..5361,5362..5384,5385..5403,5404..5426,5427..5445,
                     5446..5468,5469..5487,5488..5510,5511..5529,5530..5552,
                     5553..5571,5572..5594,5595..5613)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5646..5683
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          6583..7155
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(7287..7306)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7352..7407
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7406..7425)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7798..7815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7969..8557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8049..8068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8728..9519,9520..9588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9351..9370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9589..9693)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9761..9779
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(10353..10952)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10436..10456)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10933..10952
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(11045..11402)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11059..11194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        11211..11402
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     11359..11379
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    11406..11630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11619..11636
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tcgagttttt tttttttttt tttttatgaa aaacattgtt ttattacttg aaaaataata
       61 gaaattgtat ataaaataca aaatatacac ttgcctagtg caattggtct ccattttaca
      121 ttttttacat gttttttccc tatttaatgg cacccaaaat gcggtttcat tttcactgcc
      181 ctggtcaact aataccattt aacaacgtat ttaagaactt cttcaaaaga cagtaaaaac
      241 aatttgtaaa ataactatgc tttcttacat ttacaaaaca gaattttgat aatatatgaa
      301 tattttatat cacataatta ccatgtaact actaatctgt tatctatatc aaatcagagc
      361 acataacatt ttccaaatac actgttttaa taaagagatt agaaaacagg acagtaacag
      421 tttcttaaag caatcttaca ggaagtctaa tttaatattc tgttaggtga tgtactcacg
      481 taggctccca gaagacccag aaactacatg aattgcggaa ggctgattta agatgccagt
      541 tcttcaataa acagtgaact ttgtaattag aatgattttt aagccaaaat actactcctt
      601 actcttcaga aataaaatat agctatcctc ttataataca gcaagaaaca taattgttct
      661 ggtaattgcc agcttaacta aaaagacctc cccttttcgt tttgtccatt aatttcttgc
      721 tggtatcact tcctttggca aaccacacag gtaatcgtct ctttctctct gcagatgaag
      781 tctcagtatt gatgcctact tcttccttgc ttctcttaga acttgaacag atctcttcag
      841 aaccgggaaa acatctcctt ttgttgacat cacatgaagg ttgaagtcca ctgtcaggac
      901 catgtttaag gatctcaatt gccatgtgga taaggtacgt gtcaatgttt tcaggaacta
      961 acattctgat taggctaatt ttactcatat ctgagttgac gggagggttt cggataacat
     1021 cagcaataat cttctgaacc tctggagtca ggcctgctcg ctccaaatca agggggcagc
     1081 cagctttcac cgcttgggat aagtgcatgc caattgtcat gagaggcaga atcctgctct
     1141 cagctatgct cttcaaaggc atcttctttt cttggaataa agagtatgtg atggccatag
     1201 actgtgaaag tgtgcatatt ttattttttg ctaccagact cgtcttctgt tcttcttgag
     1261 gttttgtact tgaaaagagg tctgtctgaa cactatttgt ttggcagaaa tgtttgatga
     1321 cttccaacag aggggccaac atggcagctt tgccttcaga aacaccatca atccttttta
     1381 cgttttcaac cgtagttggt ctcattttgg ccatatccac cagtatcttg tttgttgcca
     1441 gaatagctgg gggaacatcc attttattgg catgtttctg cctagcttct accaatttgc
     1501 catataacac aatctgagtc tcctgctctt gtgccgaaat aacaggctgt gaggaactgt
     1561 aagctttttc tggtgactgt accatgatac tttttttaga aatgttactc ccagaagaaa
     1621 tcttatcaca tggtttataa gaatataact tctccaagtt agacttcttc tctgtactta
     1681 attcaactgg tacttgatta taacaatgct ctttggtgcc cgaagataca gttttcgaac
     1741 taggcagaag caacttcttt ggacacaatt cttcattagc ttgaaggatg aggctctgag
     1801 attctgtatt agctttatga agccaatttc tacccttttt cgtaagggcg caaatcttca
     1861 taaatttgtt ataccgagaa acttctacca agaatccctc agtgatcagc tgacgggaaa
     1921 aagccttcca ccaactctct gtttgatcct tgccagtgcc aaataaactg tgcctgcgat
     1981 attgatcggc aagacgctga gaattagatc ctcggagaaa taaaattgga agcccaattc
     2041 caaatttttc gcctaagatg tccacagcag acaaaagctt aaatgcttgt ggaccaaagt
     2101 cccaggatgt atcctctgag tcatccatgg aatagcaatg atccaatctg gacctgcaat
     2161 tatcacagca tttttcagtt cccataattc ccaaggaggc tttttgtact tgtttgtcct
     2221 caaaatgaga caagatgatt tgtctcctac atctgctaga atgaagatat ttttccatct
     2281 ttgccatcat ctttaatttg tataatcgaa acttctcatt acgtatctca gtaagaaggt
     2341 gcctatttaa gttaatgtct gcaggagccc agaggacgtg acaagaactt tgaagtccat
     2401 cacgaccagc tctaccaatc tcctgataat atgattccat gtccttagga gcaccgtaat
     2461 gaatgacttg gcgaatgtca gctttattaa tgcccattcc aaaagctatg gtagctatga
     2521 cacactgaat ttcatctctt acaaacctat gatgaatgtc tttccttgtg ctaaaactca
     2581 tgcccgcatg gtatgttcca caggatagat tcagtttcct aagttcacct gtaacttgtt
     2641 gtgtcatttt tctagaagga cagtagatga ttgttggacc ttcaaattcc cagtgggaac
     2701 ttgttttgac aagaaatggc tgcagatcct gaaggatatt ccctgttttt cgcctaactt
     2761 ctaaatacag gtttggtcga tcaaaaccag tacaggtgat ctgaggattt ctcagattta
     2821 agcaacgtac aatgtcttcc cggattgaag aacttgcagt agcagtaagt gcaacgattg
     2881 gaaccattgg cagtgctgtc tttagggagc ccaacttcct gaatgaatcc ctaaaatcat
     2941 gcccccactc agaaatacag tgagcctcat ccacagcaat gagcgtgata ccaatatcag
     3001 cctcaagttg ctggagcagg cccatgttac ctgaacagta ttctggagtt acgtatacaa
     3061 tccggtattt acctaattta atatctgtta gaacattttc tgactgtgct gatccaagga
     3121 agcaagctgg gatgttggac attttaagct gtagcacttg gtcttccatc agagaaataa
     3181 ggggagagat aacaaggcca atcttgccta cataaacagg tggatactgg aagcacaaac
     3241 tctttccata tccagttgcc atgacagcaa cattatctct tctttcttct aatactgaat
     3301 gaatcacttt ccactgaact ggtttaaaac tggaatggcc aaagtacatc ttgaggcaag
     3361 taacttgctc ttcattgggt gctggccaca aaaagtcctt atcatcatct tcttcccctt
     3421 cattagcttc attttcatca tcttcttctt cttctttagt aggaagaccc agatttcttt
     3481 ccatttttaa gcatttagaa tgagttggtt ctaccgtgcc actattgagg ttttctaaag
     3541 acttaagcat ctccatttct aaatcttcat cactctcaat tacataggac gtatcgtttt
     3601 cattatcatt gggagataaa tgcttaagca tctccatttc taaatcttca tcactctcaa
     3661 ttacatagga cgtatcgttt tcattatcat tgggagataa atgctcagta gatttataag
     3721 caatatcact aagatattct tcctgagact gctgttccaa aatttggagt tcatgttctg
     3781 taatatctaa cgacatcaaa caagctcttt ccatattctc tttcaatttg ttgtcttcta
     3841 ctccatcttc aaatccatct tcttttcgtt ccactttatt tccaagtaca tcttctccat
     3901 catgtttagc taaatgatca agtgttgggt cccatgtttc atcttcaacg tgaattaaaa
     3961 cttcatgttc tctaatttgt ttctgttgta ctcccccagt agttgaatct tcaaaggata
     4021 ataagtttaa attattgctg ggcctcagtt cagtctcaat gttagtagac ccaattatca
     4081 tcctcctcag tgaatataga ttttctgaaa tatcctttag taagatagaa accctccgtg
     4141 ggttttccaa tttactgaaa gcatgaggaa gatgcttagc cagatccatc acttcctcag
     4201 agattgaagt caactgtttg ttcatgtcgc taagtaggat ttcttcctct ttatttatag
     4261 caaacctttg cacagtatca tccaaaatct ctaaatttcg gtaaataata aaaccagcat
     4321 aagcatcagt ggctgcatac agtttctggt cctcagtgag aggaaattta ctccaattgc
     4381 tacagcggat agacttgtct ttcaggagct gtttacctaa gaggtgttta accagactgt
     4441 taaggctcca ggtctctgta catttcagct ttttattggc aacatctgtc aactccacaa
     4501 aattcttcaa tttgatatca aagtcacgta gaagtttcca ctgatctcct tcaattccta
     4561 cacctgcctt tttaactgct ttattttcaa gcaacatttt taatccctgg ggaaaaactg
     4621 acatggaaga aacgtggaac aagtaacatt tgctctcaga aacacacaac tgaattagtg
     4681 caactttgcc aagtttccct ctgttgtata atggaggcca cgccatgtca aatcccacca
     4741 catccccatc tgatagactc atgctaatat cttctgacag gaaagagcaa tcactagcat
     4801 cgtaactata cacaatggat ccagtgaatt ctaagaaggg gaggtcatct tcaaaaacac
     4861 tcttccgaac acatgccttt ctttcttcta cagcacatct tttattctgc acattcatcc
     4921 attcaggaca ttttcgctgc tgtgcagttg tttccaattt tttttcaccc atggtgaggt
     4981 cgcccaagct ctccatttca ttcaagtcct cttcagaaat gagcttttgc tccatttcat
     5041 tcaagtcctc ttcagaaatg agcttttgct ccatttcatt caagtcctct tcagaaatga
     5101 gcttttgctc catttcattc aagtcctctt cagaaatgag cttttgctcc atttcattca
     5161 agtcctcttc agaaatgagc ttttgctcca tagctttaaa tcgattcctg caggaattcg
     5221 gggccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg
     5281 cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggt cgagtttacc
     5341 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     5461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     5521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     5641 gtcgagtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     5701 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     5761 cagcctccgc ggccccgaat tcgagctcca ccgcggtggc ggccgccgcc accatgagca
     5821 agggcgagga actgttcact ggcgtggtcc caattctcgt ggaactggat ggcgatgtga
     5881 atgggcacaa attttctgtc agcggagagg gtgaaggtga tgccacatac ggaaagctca
     5941 ccctgaaatt catctgcacc actggaaagc tccctgtgcc atggccaaca ctggtcacta
     6001 ccttcaccta tggcgtgcag tgcttttcca gatacccaga ccatatgaag cagcatgact
     6061 ttttcaagag cgccatgccc gagggctatg tgcaggagag aaccatcttt ttcaaagatg
     6121 acgggaacta caagacccgc gctgaagtca agttcgaagg tgacaccctg gtgaatagaa
     6181 tcgagctgaa gggcattgac tttaaggagg atggaaacat tctcggccac aagctggaat
     6241 acaactataa ctcccacaat gtgtacatca tggccgacaa gcaaaagaat ggcatcaagg
     6301 tcaacttcaa gatcagacac aacattgagg atggatccgt gcagctggcc gaccattatc
     6361 aacagaacac tccaatcggc gacggccctg tgctcctccc agacaaccat tacctgtcca
     6421 cccagtctgc cctgtctaaa gatcccaacg aaaagagaga ccacatggtc ctgctggagt
     6481 ttgtgaccgc tgctgggatc acacatggca tggacgagct gtacaagtga gcggccgctc
     6541 tagcgcggcc tcgacgatat ctctagagct gagaacttca gggtgagttt ggggaccctt
     6601 gattgttctt tctttttcgc tattgaaaaa ttcatgttat atggaggggg caaagttttc
     6661 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
     6721 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
     6781 tctgtaactt ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     6841 cgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     6901 taattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     6961 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
     7021 tacatcctgg taatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
     7081 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
     7141 ttctttttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc
     7201 aaagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     7261 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     7321 atgaagcccc ttgagcatct gacttctggg taataaagga aatttatttt cattgcaata
     7381 gtgtgtggga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     7441 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca
     7501 tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7561 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7621 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7681 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaactc gactgcatta
     7741 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7861 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8161 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8401 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8641 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8701 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8821 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8881 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9721 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9781 ctttcgtctt cactcgacgc tggtcgagct gatacttccc gtccgccagg ggacatgccg
     9841 gcgatgctga aggtcgcgcg cattcccgat gaagaggccg gttaccgcct gttgacctgg
     9901 tgggacgggc agggcgccgc ccgagtcttg cctcggcggc gggcgctctg ctcatggagc
     9961 gcgcgtccgg ggaccttgca cagatagcgt ggtccggcca gacgacgagg cttgcaggat
    10021 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10081 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacaaca acaattgcat
    10141 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
    10201 ctacaaatgt ggtatggctg attatgatcc tctagagtcg gtgggcctcg ggggcgggtg
    10261 cggggtcggc ggggccgccc gggtcggctt cggtcggagc catggggtcg tgcgctcctt
    10321 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
    10381 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
    10441 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
    10501 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
    10561 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
    10621 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
    10681 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
    10741 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
    10801 tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg
    10861 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg
    10921 gcgaggcgca ccgtgggctt gtactcggtc atggaaggtc gtctccttgt gaggggtcag
    10981 gggcgtgggt caggggatgg tggcggcacc ggtcgtggcg gccgacctgc aggcatgcaa
    11041 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
    11101 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
    11161 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
    11221 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
    11281 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
    11341 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
    11401 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
    11461 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
    11521 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
    11581 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
    11641 tcagcggttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
    11701 ttctgcagat atccagcaca gtggcggccg c
//
