LOCUS       sequence-64909-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pCMV-AD-uvr8at
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             131..673
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccatgg gccctaaaaa gaagcgtaaa gtcgccatcg atcagctcac
      121 catggtgttt ccttctgggc agatctcaaa ccaggccctg gccttagcac cgtcctctgc
      181 cccagtcctt gcccagacca tggtcccttc ctcagccatg gtacctctgg ctcagccccc
      241 agctcctgcc ccagttctaa ccccgggtcc tccccagtcc ctgtctgcac ctgttccaaa
      301 gagcacccag gctggggaag gcacgctgtc ggaagccctg ctgcacctgc agtttgatgc
      361 tgatgaagac ttgggggcct tgcttggcaa cagcacagac ccaggagtgt tcacagacct
      421 ggcatctgtg gacaactcag agtttcagca gctcctgaac cagggtgtgt ccatgtctca
      481 ctccacagct gagcccatgc tgatggagta ccctgaagct ataactcgcc tggtgacagg
      541 gtcccagagg ccccctgacc cagctcccac acccctgggg acctcggggc ttcccaatgg
      601 tctctccgga gatgaagact tctcctccat tgcggacatg gacttctctg ctctgctgag
      661 tcagatcagc tccagcggcc aaggatccgc ggaggatatg gctgccgacg aagttacggc
      721 tcctcctcgt aaggttctta tcatctccgc tggtgctagc cactccgtcg ctcttctctc
      781 tggtgacatt gtttgttctt ggggtcgagg agaggatgga cagttaggtc atggcgatgc
      841 agaggatcga ccttctccga ctcagctt
//
