LOCUS       sequence-64923-e        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Boniface EJ, Lu J, Victoroff T, Zhu M, Chen W
  TITLE     FlEx-based transgenic reporter lines for visualization of Cre and 
            Flp activity in live zebrafish.
  JOURNAL   Genesis. 2009 Jul;47(7):484-91. doi: 10.1002/dvg.20526.
  PUBMED    19415631
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg"; 
            journalName: "Genesis"; date: "2009-07"; volume: "47"; issue: "7"; 
            pages: "484-91"
COMMENT     Sequence Label: pT2-eab2-[GTR]
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..828)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    856..1080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1069..1086
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    complement(1102..1135)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1182..1215)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1248..1281
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    1306..1353
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1459..2169
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1604..1622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2292..2413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2563..2684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2813..2947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2992..3705,3706..3708,3709..3711))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2995..3705,3706..3708,3709..3711))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3037..3058)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3386..3405
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3645..3666
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3708..3717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3837..3870)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(3916..3949)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    3976..4009
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4056..4089
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6361..6548,6549..6560)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6594..6610)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6594..6610)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6647..6667)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6647..6665)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6686..6702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6686..6702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6734..6740,6741..6758,6759..6764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6779..6800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6917..6934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7088..7676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7168..7187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7847..8638,8639..8707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8470..8489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8708..8812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattag atcctggttc
      841 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
      901 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
      961 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     1021 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     1081 tcgaggctga tcagcggttt cataacttcg tatagtacac attatacgaa gttatgggtc
     1141 gatggtgaga tctggactag agggtcgatg gtgatgcttg gataacttcg tatagcatac
     1201 attatacgaa gttattaggg ataacagggt aatgaagttc ctattccgaa gttcctattc
     1261 ttcaaatagt ataggaactt cgtgtgtgtg tgtgtgtgtg tgtgtgaagt tcctattccg
     1321 aagttcctat tctctagaaa gtataggaac ttctcgagac taatccggag tgtgtgtgtg
     1381 tttctgacgt gtctctctct tccctttttt ttttcaggtc gacaaactct tcgcggtcgg
     1441 aagaagagaa gaaccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     2161 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     2221 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2281 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2401 tcaatgtatc ttaaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc
     2461 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2521 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2581 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgatctgat
     2701 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2761 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2821 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2881 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2941 ctggatcatc atcgatgaat taattcgagc tccaccgcgg tggcggccgc tttacttgta
     3001 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     3061 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     3121 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     3181 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     3241 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     3301 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     3361 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     3421 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     3481 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     3541 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     3601 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     3661 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     3721 cggtggatcc gtcgaggaat tctttgccaa aatgatgaga cagcacaaca accagcacgt
     3781 tgcccaggag ctgtaggaaa gagaagaagg catgaacatg gttagcagag ggatccgaag
     3841 ttcctatact atttgaagaa taggaacttc ggaataggaa cttcctggcc ttgggggagg
     3901 gggaggccag aatgagaagt tcctatactt tctagagaat aggaacttct aactataacg
     3961 gtcctaaggt agcgaataac ttcgtataat gtgtactata cgaagttatg ggtcgatggt
     4021 gatgcttggc aattcgggtc gatggtgaag cttggataac ttcgtataat gtatgctata
     4081 cgaagttatg ctagctaaag taggtcacac cctgctgcag cactgcctgc acaaagaact
     4141 tcccatgccg gctatccatc aagtgtgcaa catgctcacc agcaccctca aaactgtagt
     4201 taaaaatcac atttatggct gttaaagaga gattagccag aacacaactt tatattaaga
     4261 cttaacaccc atttcacacc ggcctattca atgttcaatg tcgtgcctac gactggtcac
     4321 gttaaattac cgacattctc acctgccaca tccactaacg ctacaaaaaa gttaagatac
     4381 agccaatatt tattaaatgt aacatagatt atcaaaagaa ccaccactta taaatcaaac
     4441 ctagtagata actacaaaga gaccgttatg aactgtctaa agacttcatc aaatcatatt
     4501 tatccattta cacattttac ttaacattaa tagatagaaa ctataattaa acgcttaaaa
     4561 actgtaaaac caactcacct tgggtttggg gggttgtcga cttaagagaa tgggttacaa
     4621 gcagatgata ttccgcacta gctgcgtgtg gaggagctca aagtgaggct gaaaagctgg
     4681 gcgcgctctt ttatatgacg gcacataccc ggctctagcc ataaaaggta aactttcgga
     4741 gctctgtgct ctgattggtc cagcgtcaca cacccggctg tcgcgtgcac attgctacac
     4801 ttgcttcttg caacttgaga gtgaaagaag gggaagctta actggttaaa gaacaaggcc
     4861 atggcgattt cctataaaac tggaattttt atttgcaaat tttaacacag aatttttcgt
     4921 atttttctgc gacaacgcca agactcaatt aaacttttca aaaagtctgt gcagagttac
     4981 aagtggcaga atgcgtcata atgatggatg cctccatgat cctgtgttgt gttttcaatc
     5041 taacacaaca gcagctctgt aggtcattta cctcttgtgt tcacgaaata ccgtaataca
     5101 aacaatgggg gatctaatga gtacttttcc atgtataaaa tggacatttt ttttatccaa
     5161 attaggatga cactacttgt agtatgttgt atgagcaact agctggtctt agctcagtag
     5221 taatgcactt taagaaagtg ctttaataac tttgaatgaa tgggacctgc attccatgcc
     5281 acattgcttc actatttttg tgtgtacaag ttacacaatg atgtataaag tgagtgatag
     5341 cagttacatt tttgcaagtg ggcctacctc aggtttaatg ttcattagaa atgttatatg
     5401 tcaagtcttg atcttaaata gttaaatttt ctgttcataa tttcatgtcg tgtctatgca
     5461 gacataacat ttctttttaa agaggtttat aaggataaat cctaagaaca taattctcat
     5521 taaaagtaga aaaaaaaaac actgaaagtg ttggtgatca gctacaataa ataatatttt
     5581 gcacacattt cagatattgc cacactgaat aacagataaa gctgtgcttg agcagcagtg
     5641 atcatgataa actacatgtg actttttact gtgtaacttg ggagtaatgc acatggcctt
     5701 cagtgggagt tataaacaaa aacagctgat tgtgttaaaa gtgcttgctg ataccaagga
     5761 caatgaggtg atgtgacttt tattttgttt ttggcaaatt ccaagtattt tgacaagact
     5821 ttttacaatt ctgagcattt tacctctaaa atatggaagt ttctcagagt aaacgttgag
     5881 tataggtaat gactaggccc acacagaatc tgtgcgcaca gaaatctgca gatttcccca
     5941 gatctttagc ccatcattga gtttatttat tgacttgtgt atttggattt gctctttagt
     6001 gtttggactc tcagcagtaa aaattaactc acactgaact aaactgaact gaacttaaac
     6061 tcttaaaaaa tggactgaca cagtttcaat ttactatact aatcctctaa gttaagctgc
     6121 tttgacacaa tctacattgt aaaagcactc tagcttgcat gcctgagaat ttcagaatgt
     6181 aatgatacct ttgttagata aggctgttta catctgatag tggaccttaa gccgacactt
     6241 aaatgataaa aacggcaaag aattgcaagt ttgatttgca ttggaaaggg tcgctggctt
     6301 ttgtgttaca cgccccttat ttgtgcttga ttagatgatc cccctgctcg agccgggccc
     6361 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
     6421 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
     6481 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
     6541 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
     6601 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6661 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6841 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7501 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7981 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8821 cccgaaaagt g
//
