LOCUS       sequence-64930-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     pIRES-EGFP-puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIRES-EGFP-puro
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(119..693,694..696,697..705)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(286..303)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     513..532
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(751..772)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tcgatccgcc
      121 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
      181 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
      241 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
      301 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
      361 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
      421 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
      481 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
      541 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
      601 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
      661 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
      721 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      781 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      841 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      901 acctac
//
