LOCUS       sequence-64955-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     Akt
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1236 pcDNA3 Myr HA Akt3
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..143
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             147..173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttcccaccac catggggagc agcaagagca
      121 agcccaagga ccccagccag cgcgcctacg cctacgacgt gcccgactac gcctccctcg
      181 gatctatgag cgatgttacc attgtgaaag aaggttgggt tcagaagagg ggagaatata
      241 taaaaaactg gaggccaaga tacttccttt tgaagacaga tggctcattc ataggatata
      301 aagagaaacc tcaagatgtg gatttacctt atcccctcaa caacttttca gtggcaaaat
      361 gccagttaat gaaaacagaa cgaccaaagc caaacacatt tataatcaga tgtctccagt
      421 ggactactgt tatagagaga acatttcatg tagatactcc agaggaaagg gaagaatgga
      481 cagaagctat ccaggctgta gcagacagac tgcagaggca agaagaggag agaatgaatt
      541 gtagtccaac ttcacaaatt gataatatag gagaggaaga gatggatgcc tctacaaccc
      601 atcataaaag aaagacaatg aatgattttg actatttgaa actactaggt aaaggcactt
      661 ttgggaaagt tattttggtt cgagagaagg caagtggaaa atactatgct atgaagattc
      721 tgaagaaaga agtcattatt gcaaaggatg aagtggcaca cactctaact gaaagcagag
      781 tattaaagaa cactagacat ccctttttaa catccttgaa atattccttc cagacaaaag
      841 accgtttgtg ttttgtgatg gaatatgtta atgggggcga gctgtttttc catttgtcga
      901 gagagcgggt gttctctgag gaccgcacac gtttctatgg tgcagaaatt gtctctgcct
      961 tggactatct acattc
//
