LOCUS       sequence-64972-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTGR2
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 accttaagaa ggagatatac tatgatgatt gttcaaagag tggtattgaa ttctcgacct
      181 ggaaaaaatg gtaatccagt ggcagagaat ttccgaatgg aagaagtcta tttaccagat
      241 aatattaatg aaggacaagt acaagttaga actctttatc tttctgtgga tccttacatg
      301 cgttgtagaa tgaatgaaga cactggcact gattatataa caccttggca gctatctcaa
      361 gtcgttgatg gtggaggtat tggaattata gaagaaagca aacacacaaa tttgactaaa
      421 ggcgattttg tgacttcttt ctattggccc tggcaaacca aggttattct ggatggaaat
      481 agccttgaaa aggtagaccc acaacttgtg gatggacacc tttcatattt tcttggagct
      541 ataggtatgc ctggtttgac ttccttgatt gggatacagg aaaaaggtca tataactgct
      601 ggatctaata agacaatggt tgtcagtggg gccgcaggtg cctgtggatc tgtggctggg
      661 cagattggcc atttcttagg ttgttccaga gtggtgggaa tttgtggaac acatgagaaa
      721 tgcatcctct tgacctcaga actgggcttt gatgctgcaa ttaattataa aaaagacaat
      781 gtggcagaac agctccgtga atcatgccca gctggagtgg atgtttattt tgacaatgtt
      841 ggtggtaaca tcagtgatac agtgataagt cagatgaatg agaacagcca catcatcctg
      901 tgtggtcaaa tttctcagta caacaaagat gtgccttatc ctcccccgct atcccctgct
      961 atagaggcaa tccagaaaga aagaaacatc acaagggaaa gatt
//
