LOCUS       sequence-64973-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTGR2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgcgatttct tctgaaatgc
      181 aaactatctg ctttccaatg ttacctcctg tcatcatgga ctggaatgca gctcccatgt
      241 tttccaaccc atttattacc gtctctttaa tctttagctt tccttcttta aaccactgac
      301 tcagctgtag aatgccaggc tcaaatttgt ctttataatt taataccaga aatctttccc
      361 ttgtgatgtt tctttctttc tggattgcct ctatagcagg ggatagcggg ggaggataag
      421 gcacatcttt gttgtactga gaaatttgac cacacaggat gatgtggctg ttctcattca
      481 tctgacttat cactgtatca ctgatgttac caccaacatt gtcaaaataa acatccactc
      541 cagctgggca tgattcacgg agctgttctg ccacattgtc ttttttataa ttaattgcag
      601 catcaaagcc cagttctgag gtcaagagga tgcatttctc atgtgttcca caaattccca
      661 ccactctgga acaacctaag aaatggccaa tctgcccagc cacagatcca caggcacctg
      721 cggccccact gacaaccatt gtcttattag atccagcagt tatatgacct ttttcctgta
      781 tcccaatcaa ggaagtcaaa ccaggcatac ctatagctcc aagaaaatat gaaaggtgtc
      841 catccacaag ttgtgggtct accttttcaa ggctatttcc atccagaata accttggttt
      901 gccagggcca atagaaagaa gtcacaaaat cgcctttag
//
