LOCUS       sequence-64981-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: INCENP
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(327..349)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(494..512)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        580..684
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(903..922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatcgagct cagagaacct gtacttccaa tccatggagg cccatccccg gaagcccatc
       61 cccacctggg cccgaggcac cccgctcagc caggctatca ttcaccagta ctaccaccca
      121 ccgaaccttc tggagctctt tggaaccatt ctcccactgg acttggagga tatcttcaag
      181 aagagcaagc cccgctatca caagcgcacc agctctgctg tctggaactc accgcccctg
      241 cagtgacagt aaaggtggat actcgagcgg ccgcatcgtg actgactgac gatctgcctc
      301 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
      361 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
      421 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct
      481 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      541 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      901 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagaacaact cggtcgccgc
      961 atacactat
//
