LOCUS       sequence-64982-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Hackel BJ, Kapila A, Wittrup KD
  TITLE     Picomolar affinity fibronectin domains engineered utilizing loop 
            length diversity, recursive mutagenesis, and loop shuffling.
  JOURNAL   J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 
            10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24.
  PUBMED    18602401
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2008.06.051"; journalName: "J Mol Biol"; date: 
            "2008-09-19- 19"; volume: "381"; issue: "5"; pages: "1238-52"
COMMENT     Sequence Label: pCT-L7.5.1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..124
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             456..467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             468..494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             861..890
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
       61 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
      121 cgtcaaggag aaaaaacccc ggatcgaatt ccctacttca tacattttca attaagatgc
      181 agttacttcg ctgtttttca atattttctg ttattgcttc agttttagca caggaactga
      241 caactatatg cgagcaaatc ccctcaccaa ctttagaatc gacgccgtac tctttgtcaa
      301 cgactactat tttggccaac gggaaggcaa tgcaaggagt ttttgaatat tacaaatcag
      361 taacgtttgt cagtaattgc ggttctcacc cctcaacaac tagcaaaggc agccccataa
      421 acacacagta tgtttttaag gacaatagct cgacgattga aggtagatac ccatacgacg
      481 ttccagacta cgctctgcag gctagtggtg gtggtggttc tggtggtggt ggttctggtg
      541 gtggtggttc tgctagcgtt tctgatgttc cgagggacct ggaagttgtt gctgcgacct
      601 ccaccagcct actgatcagt tggcgcggct acccctgggc tacctattat gggatcattt
      661 acggagaaac gggaggaaat agccttgtcc aggagttcac tatgcctggg gttaccaatg
      721 ctaccatcag cggccttaaa cctggagttg attataccat cactgtgtat gctgtcactc
      781 gcgtggggcg gacgtttgac acgccgggcc caatctccat taattaccga acagaaattg
      841 acaaaccatc ccagggatcc gaacaaaagc ttatttctga agaggacttg taatagctcg
      901 agatctgata acaacagtgt agatgtaaca aaatcgactt tgttcccact g
//
