LOCUS       sequence-64995-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Hackel BJ, Kapila A, Wittrup KD
  TITLE     Picomolar affinity fibronectin domains engineered utilizing loop 
            length diversity, recursive mutagenesis, and loop shuffling.
  JOURNAL   J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 
            10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24.
  PUBMED    18602401
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2008.06.051"; journalName: "J Mol Biol"; date: 
            "2008-09-19- 19"; volume: "381"; issue: "5"; pages: "1238-52"
COMMENT     Sequence Label: pCT-L7.5.1
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(348..377)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(744..770)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(771..782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcaa ttctcttagg attcgattca cattcatctt
       61 tttttagcta ctaataggat aaattatagg aatttataac aaattgaaat atggcaggca
      121 gcaaaattaa aattgtctta gttttttagt gtatagaagt gaatagctat ataaagtatg
      181 tgtaaagttg gtaacggaac gaaaaataga aaaggatatt acatgggaaa acatgttgtt
      241 tacggagaaa tgaaaagtat attgtatttt gtacgagcta aaagtacagt gggaacaaag
      301 tcgattttgt tacatctaca ctgttgttat cagatctcga gctattacaa gtcctcttca
      361 gaaataagct tttgttcgga tccctgggat ggtttgtcaa tttctgttcg gtaattaatg
      421 gagattgggc ccggcgtgtc aaacgtccgc cccacgcgag tgacagcata cacagtgatg
      481 gtataatcaa ctccaggttt aaggccgctg atggtagcat tggtaacccc aggcatagtg
      541 aactcctgga caaggctatt tcctcccgtt tctccgtaaa tgatcccata ataggtagcc
      601 caggggtagc cgcgccaact gatcagtagg ctggtggagg tcgcagcaac aacttccagg
      661 tccctcggaa catcagaaac gctagcagaa ccaccaccac cagaaccacc accaccagaa
      721 ccaccaccac cactagcctg cagagcgtag tctggaacgt cgtatgggta tctaccttca
      781 atcgtcgagc tattgtcctt aaaaacatac tgtgtgttta tggggctgcc tttgctagtt
      841 gttgaggggt gagaaccgca attactgaca aacgttactg atttgtaata ttcaaaaact
      901 ccttgcattg ccttcccgtt ggccaaaata gtagtcgttg ac
//
