LOCUS       sequence-65008-e       12351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-5-G4BDMD-QFADM1-hsp70
FEATURES             Location/Qualifiers
     source          1..12351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6770..7210
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    9875..9944
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(10163..10181)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10202..10218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10202..10218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10215..10237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10226..10242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10250..10256,10257..10274,10275..10280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10295..10316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10433..10450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10604..11192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10684..10703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11363..12154,12155..12223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11986..12005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12224..12328)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgacctc
     4861 aaagatggaa gcggagcaga aggagcgatt tcagacagac gcccccaagt tttgggattc
     4921 cttctacggc attcacgaca accgcttctt caaggatcgc cactggctgt ttacggagtt
     4981 ccccgaacta gctcctctgg cggcggattc ggcggtactg caaccacgta gcattttcga
     5041 actgggctgt ggagtaggaa acacaatttt accccttcta cagtacagct ctgaaccgca
     5101 gctcaaggtt ttcggatgtg acttctccgc ccgggccatt gaaatcctgc gaagccaacg
     5161 gcagttcgac gagaagcgct gcgaggtgtt tgtcatggat gccacgctgg atcattggca
     5221 ggtgcccttc gaggaaaact cacaggacat tattgtgatg atttttgtgc tgtccgcaat
     5281 tgagccaaag aaaatgcagc gggtattgga caattgctat cgctacctgc gtcccggtgg
     5341 cttgctcctc ttccgcgact acggacgata cgacctggca cagctgcgct tcaagagcgg
     5401 caagtgtatg gaggacaact tctacgtgcg aggcgacggc accatggtgt acttcttcac
     5461 ggaggaagaa ctgcggggta tgatgactca agcggggctg caggaggagc agctcattgt
     5521 ggaccgaaga ctgcaagtta atcgctgtcg cggcttgaaa atgtaccgcg tgtggattca
     5581 gacaaagttc aggaaaccgc tttaaatcaa tttagacgaa tagttctgtg cattttaaac
     5641 cttgttcaaa tcaattgata taattattta cacgatatat ttaaaaatac aaagttaaaa
     5701 cttcaaactg taatattact agaactttga gcatttcttt ttcttgatgg aaagcccggc
     5761 ttgtttttaa atttcccacc ccttggccat cggcacttgg aaatcgctaa ggaaaagctt
     5821 ctcgctggaa caccattcgc tgctggccat ccaggcagct ttttccccaa atacacataa
     5881 cagttaggaa agctgcaagt tttcgcagtt ggccaaaaac gtcgtaagtg aaaattttcc
     5941 tggtgctgcg taaaaaaatt cgtaaaatat tagttaagcc tggtttttgt aaactcaagt
     6001 tgcccgagca aaagtaaagt gattctgatc aaaagtgcta aaactgattg attaggaaaa
     6061 cgaagatcaa acgaaatgtc aaccaaagtc agcgaaaacc cttgtcgtgc agaatttcat
     6121 tgattttttc ttctgagccc acgttggcca gttgggcact tgaaattatt tttaaaacgt
     6181 tttgcatttt gaagacatct taggtgcccc tgggggtatg caacggaaat gaggaaggac
     6241 atgcctcatg gctgggcgtg ttgctgccac ccaaatagtc ctcttacgtc ccaattagaa
     6301 tcccatagct gcccatttca agcattaacc gatgtactaa aaatagttgc aactattttc
     6361 gcactctatt cagcagcagt tccagcgctg agccagaaaa ttcctgcgaa aggctgtcgt
     6421 ggcaagtgtt acccgcacac atacacatca tccagatcca gccaatcccc ccgtggagtg
     6481 gagtgaattt tcattgacga cggggcgaaa aagctctggc ggaagagcga cagagagaga
     6541 gagaaacaga gagagagaga gagaagcggc actgagagcg caaccctcta gaacaaaaac
     6601 aacgaaacag agttcagctg caccaccacc cactacctac accaccaacg ccaccaccgc
     6661 ccactgacca tcccacatcc cgacctgcgc cagtggaaga cagtgaaaga ggccaagtgc
     6721 atcgtgcata gagtaccttc atcctaatca tcgacagccg aattccaaca tgaagctact
     6781 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
     6841 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
     6901 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
     6961 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga
     7021 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa
     7081 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca
     7141 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt
     7201 gactgtatcg attgactcgg cagctcatca tgataactcc acaattccgt tggattttat
     7261 gcccagggat gctcttcatg gatttgattg gtctgaagag gatgacatgt cggatggctt
     7321 gcccttcctg aaaacggacc ccaacaataa tgggttcttt ggcgacggtt ctctcttatg
     7381 tattcttcga tctattggct ttaaaccgga aaattacacg aactctaacg ttaacaggct
     7441 cccgaccatg attacggata gatacacgtt ggcttctaga tccacaacat cccgtttact
     7501 tcaaagttat ctcaataatt ttcaccccta ctgccctatc gtgcactcac cgacgctaat
     7561 gatgttgtat aataaccaga ttgaaatcgc gtcgaaggat caatggcaaa tcctttttaa
     7621 ctgcatatta gccattggag cctggtgtat agagggggaa tctactgata tagatgtttt
     7681 ttactatcaa aatgctaaat ctcatttgac gagcaaggtc ttcgagtcag gttccataat
     7741 tttggtgaca gccctacatc ttctgtcgcg atatacacag tggaggcaga aaacaaatac
     7801 tagctataat tttcacagct tttccataag aatggccata tcattgggct tgaataggga
     7861 cctcccctcg tccttcagtg atagcagcat tctggaacaa agacgccgaa tttggtggtc
     7921 tgtctactct tgggagatcc aattgtccct gctttatggt cgatccatcc agctttctca
     7981 gaatacaatc tccttccctt cttctgtcga cgatgtgcag cgtaccacaa caggtcccac
     8041 catatatcat ggcatcattg aaacagcaag gctcttacaa gttttcacaa aaatctatga
     8101 actagacaaa acagtaactg cagaaaaaag tcctatatgt gcaaaaaaat gcttgatgat
     8161 ttgtaatgag attgaggagg tttcgagaca ggcaccaaag tttttacaaa tggatatttc
     8221 caccaccgct ctaaccaatt tgttgaagga acacccttgg ctatccttta caagattcga
     8281 actgaagtgg aaacagttgt ctcttatcat ttatgtatta agagattttt tcactaattt
     8341 tacccagaaa aagtcacaac tagaacagga tcaaaatgat catcaaagtt atgaagttaa
     8401 acgatgctcc atcatgttaa gcgatgcagc acaaagaact gttatgtctg taagtagcta
     8461 tatggacaat cataatgtca ccccatattt tgcctggaat tgttcttatt acttgttcaa
     8521 tgcagtccta gtacccataa agactctact ctcaaactca aaatcgaatg ctgagaataa
     8581 cgagaccgca caattattac aacaaattaa cactgttctg atgctattaa aaaaactggc
     8641 cacttttaaa atccagactt gtgaaaaata cattcaagta ctggaagagg tatgtgcgcc
     8701 gtttctgtta tcacagtgtg caatcccatt accgcatatc agttataaca atagtaatgg
     8761 tagcgccatt aaaaatattg tcggttctgc aactatcgcc caatacccta ctcttccgga
     8821 ggaaaatgtc aacaatatca gtgttaaata tgtttctcct ggctcagtag ggccttcacc
     8881 tgtgccattg aaatcaggag caagtttcag tgatctagtc aagctgttat ctaaccgtcc
     8941 accctctcgt aactctccag tgacaatacc aagaagcaca ccttcgcatc gctcagtcac
     9001 gccttttcta gggcaacagc aacagctgca atcattagtg cgtcagttgg agctccctcc
     9061 taccgccacg gctacggcct cgataatgcc gcacgtgatg gagcagcctc tcagtaccag
     9121 cattaacccc gtcaacgacc gcttcaacgg tattcccaac cccactccgt ataactccga
     9181 tgcagctctc gatgctatca ctcagaccaa cgattatgga agcgtaaata cacatggtat
     9241 cctctctact tacccgccac cggctacgca ccttaatgaa gcttccgtcg ctctcgctcc
     9301 cggtggcgcc cccccccgac cgcctcctcc gtatgttgac agcacgacca atcacccgcc
     9361 gtaccactcg aatctggttc caatggcgaa ctttggttac tcgaccgttg attacgatgc
     9421 catggttgac gatttggcta gcattgaata cacggacgct gtggatgtcg acccacagtt
     9481 tatgaccaat ctgggattcg ttcctggatg taacttctcc gacattaata catacaaaaa
     9541 gaagaaatga tgacgtctat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt
     9601 cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt
     9661 aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat
     9721 tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca
     9781 gggagtgagt ttgcttaaaa actcgtttag atccgcggcc gcggctcgag ggtacctcta
     9841 gagatccact agtgtcgacg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
     9901 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatga
     9961 acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc
    10021 cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg
    10081 gtgcggatac gcggggcagc gtcagcgggt tctcgacggt cacggcgggc atgtcgacac
    10141 tagttctagc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
    10201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
