LOCUS       sequence-65019-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wang L, Brugge JS, Janes KA
  TITLE     Intersection of FOXO- and RUNX1-mediated gene expression programs in
            single breast epithelial cells during morphogenesis and tumor 
            progression.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):E803-12. doi: 
            10.1073/pnas.1103423108. Epub 2011 Aug 22.
  PUBMED    21873240
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1103423108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-10-4- 4"; volume: "108"; issue: "40"; pages: "E803-12"
COMMENT     Sequence Label: pBabe RFP-DN-FOXO1-HA neo
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..674
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             3..674
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(130..148)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     398..417
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     592..615
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
       61 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      121 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      181 tgtcccctca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      241 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      301 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      361 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      421 tgggctggga ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga
      481 tcaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
      541 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
      601 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
      661 actccaccgg cgccctcgag ggatccatgg ccgaggcgcc tcaggtggtg gagatcgacc
      721 cggacttcga gccgctgccc cggccgcgct cgtgcacctg gccgctgccc aggccggagt
      781 ttagccagtc caactcggcc acctccagcc cggcgccgtc gggcagcgcg gctgccaacc
      841 ccgacgccgc ggcgggcctg ccctcggcct cggctgccgc tgtcagcgcc gacttcatga
      901 gcaacctgag cttgctggag gagagcgagg acttcccgca ggcgcccggc tcc
//
