LOCUS       sequence-65023-e       10719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-14-LEXA-QF-hsp70
FEATURES             Location/Qualifiers
     source          1..10719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6776..6796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6806..7057,7058..7411)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    8243..8312
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8531..8549)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8570..8586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8570..8586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8583..8605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8594..8610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8618..8624,8625..8642,8643..8648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8663..8684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8801..8818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8972..9560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9052..9071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9731..10522,10523..10591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10354..10373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10592..10696)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgacctc
     4861 aaagatggaa gcggagcaga aggagcgatt tcagacagac gcccccaagt tttgggattc
     4921 cttctacggc attcacgaca accgcttctt caaggatcgc cactggctgt ttacggagtt
     4981 ccccgaacta gctcctctgg cggcggattc ggcggtactg caaccacgta gcattttcga
     5041 actgggctgt ggagtaggaa acacaatttt accccttcta cagtacagct ctgaaccgca
     5101 gctcaaggtt ttcggatgtg acttctccgc ccgggccatt gaaatcctgc gaagccaacg
     5161 gcagttcgac gagaagcgct gcgaggtgtt tgtcatggat gccacgctgg atcattggca
     5221 ggtgcccttc gaggaaaact cacaggacat tattgtgatg atttttgtgc tgtccgcaat
     5281 tgagccaaag aaaatgcagc gggtattgga caattgctat cgctacctgc gtcccggtgg
     5341 cttgctcctc ttccgcgact acggacgata cgacctggca cagctgcgct tcaagagcgg
     5401 caagtgtatg gaggacaact tctacgtgcg aggcgacggc accatggtgt acttcttcac
     5461 ggaggaagaa ctgcggggta tgatgactca agcggggctg caggaggagc agctcattgt
     5521 ggaccgaaga ctgcaagtta atcgctgtcg cggcttgaaa atgtaccgcg tgtggattca
     5581 gacaaagttc aggaaaccgc tttaaatcaa tttagacgaa tagttctgtg cattttaaac
     5641 cttgttcaaa tcaattgata taattattta cacgatatat ttaaaaatac aaagttaaaa
     5701 cttcaaactg taatattact agaactttga gcatttcttt ttcttgatgg aaagcccggc
     5761 ttgtttttaa atttcccacc ccttggccat cggcacttgg aaatcgctaa ggaaaagctt
     5821 ctcgctggaa caccattcgc tgctggccat ccaggcagct ttttccccaa atacacataa
     5881 cagttaggaa agctgcaagt tttcgcagtt ggccaaaaac gtcgtaagtg aaaattttcc
     5941 tggtgctgcg taaaaaaatt cgtaaaatat tagttaagcc tggtttttgt aaactcaagt
     6001 tgcccgagca aaagtaaagt gattctgatc aaaagtgcta aaactgattg attaggaaaa
     6061 cgaagatcaa acgaaatgtc aaccaaagtc agcgaaaacc cttgtcgtgc agaatttcat
     6121 tgattttttc ttctgagccc acgttggcca gttgggcact tgaaattatt tttaaaacgt
     6181 tttgcatttt gaagacatct taggtgcccc tgggggtatg caacggaaat gaggaaggac
     6241 atgcctcatg gctgggcgtg ttgctgccac ccaaatagtc ctcttacgtc ccaattagaa
     6301 tcccatagct gcccatttca agcattaacc gatgtactaa aaatagttgc aactattttc
     6361 gcactctatt cagcagcagt tccagcgctg agccagaaaa ttcctgcgaa aggctgtcgt
     6421 ggcaagtgtt acccgcacac atacacatca tccagatcca gccaatcccc ccgtggagtg
     6481 gagtgaattt tcattgacga cggggcgaaa aagctctggc ggaagagcga cagagagaga
     6541 gagaaacaga gagagagaga gagaagcggc actgagagcg caaccctcta gaacaaaaac
     6601 aacgaaacag agttcagctg caccaccacc cactacctac accaccaacg ccaccaccgc
     6661 ccactgacca tcccacatcc cgacctgcgc cagtggaaga cagtgaaaga ggccaagtgc
     6721 atcgtgcata gagtaccttc atcctaatca tcgacagccg aattccaaaa tgccacccaa
     6781 gaagaagcga aaagtagaag atccaatgaa ggctctcacg gcccgacaac aggaagtttt
     6841 tgatttgata cgggatcata tatcccaaac gggtatgcct ccgacccgcg cagagatagc
     6901 acagcgactg ggctttcgat cgcctaacgc cgcggaggag cacttgaagg cactggcccg
     6961 caagggtgtc attgaaatcg tgtccggtgc gagccgcgga atccggctgt tgcaggagga
     7021 ggaagagggc ctgccactgg tgggacgcgt ggccgctggc gagccgctgc tggcccagca
     7081 acacatagag ggacactatc aggtggaccc ctccttgttt aagccaaatg ctgatttcct
     7141 gttgcgggtg tcgggaatgt ccatgaagga catcggtatt atggatggtg acctcctcgc
     7201 cgtccataag acacaggatg tcaggaacgg ccaggtagtc gttgccagga tagacgatga
     7261 ggtcactgtg aaacgtctca agaagcaagg caataaggtc gagctgctgc cggagaatag
     7321 cgagttcaag ccgatcgtgg tggatctgcg acagcagtcc tttactatcg agggcttggc
     7381 cgtgggtgtg atccgcaacg gagattggct gggacgtcag ttggagctcc ctcctaccgc
     7441 cacggctacg gcctcgataa tgccgcacgt gatggagcag cctctcagta ccagcattaa
     7501 ccccgtcaac gaccgcttca acggtattcc caaccccact ccgtataact ccgatgcagc
     7561 tctcgatgct atcactcaga ccaacgatta tggaagcgta aatacacatg gtatcctctc
     7621 tacttacccg ccaccggcta cgcaccttaa tgaagcttcc gtcgctctcg ctcccggtgg
     7681 cgcccccccc cgaccgcctc ctccgtatgt tgacagcacg accaatcacc cgccgtacca
     7741 ctcgaatctg gttccaatgg cgaactttgg ttactcgacc gttgattacg atgccatggt
     7801 tgacgatttg gctagcattg aatacacgga cgctgtggat gtcgacccac agtttatgac
     7861 caatctggga ttcgttcctg gatgtaactt ctccgacatt aatacatacg aacagtgatg
     7921 acgtctatcg ataccgtcga ctaaagccaa atagaaaatt attcagttcc tggcttaagt
     7981 ttttaaaagt gatattattt atttggttgt aaccaaccaa aagaatgtaa ataactaata
     8041 cataattatg ttagttttaa gttagcaaca aattgatttt agctatatta gctacttggt
     8101 taataaatag aatatattta tttaaagata attgcgtttt tattgtcagg gagtgagttt
     8161 gcttaaaaac tcgtttagat ccgcggccgc ggctcgaggg tacctctaga gatccactag
     8221 tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
     8281 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt
     8341 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc
     8401 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc
     8461 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca
     8521 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     8581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10681 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
