LOCUS       sequence-65024-e       12561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70
FEATURES             Location/Qualifiers
     source          1..12561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6776..6796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6806..7057,7058..7411)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    10085..10154
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(10373..10391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10412..10428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10412..10428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10425..10447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10436..10452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10460..10466,10467..10484,10485..10490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10505..10526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10643..10660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10814..11402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10894..10913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11573..12364,12365..12433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12196..12215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12434..12538)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgacctc
     4861 aaagatggaa gcggagcaga aggagcgatt tcagacagac gcccccaagt tttgggattc
     4921 cttctacggc attcacgaca accgcttctt caaggatcgc cactggctgt ttacggagtt
     4981 ccccgaacta gctcctctgg cggcggattc ggcggtactg caaccacgta gcattttcga
     5041 actgggctgt ggagtaggaa acacaatttt accccttcta cagtacagct ctgaaccgca
     5101 gctcaaggtt ttcggatgtg acttctccgc ccgggccatt gaaatcctgc gaagccaacg
     5161 gcagttcgac gagaagcgct gcgaggtgtt tgtcatggat gccacgctgg atcattggca
     5221 ggtgcccttc gaggaaaact cacaggacat tattgtgatg atttttgtgc tgtccgcaat
     5281 tgagccaaag aaaatgcagc gggtattgga caattgctat cgctacctgc gtcccggtgg
     5341 cttgctcctc ttccgcgact acggacgata cgacctggca cagctgcgct tcaagagcgg
     5401 caagtgtatg gaggacaact tctacgtgcg aggcgacggc accatggtgt acttcttcac
     5461 ggaggaagaa ctgcggggta tgatgactca agcggggctg caggaggagc agctcattgt
     5521 ggaccgaaga ctgcaagtta atcgctgtcg cggcttgaaa atgtaccgcg tgtggattca
     5581 gacaaagttc aggaaaccgc tttaaatcaa tttagacgaa tagttctgtg cattttaaac
     5641 cttgttcaaa tcaattgata taattattta cacgatatat ttaaaaatac aaagttaaaa
     5701 cttcaaactg taatattact agaactttga gcatttcttt ttcttgatgg aaagcccggc
     5761 ttgtttttaa atttcccacc ccttggccat cggcacttgg aaatcgctaa ggaaaagctt
     5821 ctcgctggaa caccattcgc tgctggccat ccaggcagct ttttccccaa atacacataa
     5881 cagttaggaa agctgcaagt tttcgcagtt ggccaaaaac gtcgtaagtg aaaattttcc
     5941 tggtgctgcg taaaaaaatt cgtaaaatat tagttaagcc tggtttttgt aaactcaagt
     6001 tgcccgagca aaagtaaagt gattctgatc aaaagtgcta aaactgattg attaggaaaa
     6061 cgaagatcaa acgaaatgtc aaccaaagtc agcgaaaacc cttgtcgtgc agaatttcat
     6121 tgattttttc ttctgagccc acgttggcca gttgggcact tgaaattatt tttaaaacgt
     6181 tttgcatttt gaagacatct taggtgcccc tgggggtatg caacggaaat gaggaaggac
     6241 atgcctcatg gctgggcgtg ttgctgccac ccaaatagtc ctcttacgtc ccaattagaa
     6301 tcccatagct gcccatttca agcattaacc gatgtactaa aaatagttgc aactattttc
     6361 gcactctatt cagcagcagt tccagcgctg agccagaaaa ttcctgcgaa aggctgtcgt
     6421 ggcaagtgtt acccgcacac atacacatca tccagatcca gccaatcccc ccgtggagtg
     6481 gagtgaattt tcattgacga cggggcgaaa aagctctggc ggaagagcga cagagagaga
     6541 gagaaacaga gagagagaga gagaagcggc actgagagcg caaccctcta gaacaaaaac
     6601 aacgaaacag agttcagctg caccaccacc cactacctac accaccaacg ccaccaccgc
     6661 ccactgacca tcccacatcc cgacctgcgc cagtggaaga cagtgaaaga ggccaagtgc
     6721 atcgtgcata gagtaccttc atcctaatca tcgacagccg aattccaaaa tgccacccaa
     6781 gaagaagcga aaagtagaag atccaatgaa ggctctcacg gcccgacaac aggaagtttt
     6841 tgatttgata cgggatcata tatcccaaac gggtatgcct ccgacccgcg cagagatagc
     6901 acagcgactg ggctttcgat cgcctaacgc cgcggaggag cacttgaagg cactggcccg
     6961 caagggtgtc attgaaatcg tgtccggtgc gagccgcgga atccggctgt tgcaggagga
     7021 ggaagagggc ctgccactgg tgggacgcgt ggccgctggc gagccgctgc tggcccagca
     7081 acacatagag ggacactatc aggtggaccc ctccttgttt aagccaaatg ctgatttcct
     7141 gttgcgggtg tcgggaatgt ccatgaagga catcggtatt atggatggtg acctcctcgc
     7201 cgtccataag acacaggatg tcaggaacgg ccaggtagtc gttgccagga tagacgatga
     7261 ggtcactgtg aaacgtctca agaagcaagg caataaggtc gagctgctgc cggagaatag
     7321 cgagttcaag ccgatcgtgg tggatctgcg acagcagtcc tttactatcg agggcttggc
     7381 cgtgggtgtg atccgcaacg gagattggct gggatccatc gacagtgcgg cccaccacga
     7441 caacagcacc attccactgg acttcatgcc tcgtgacgca ctgcatggat ttgactggag
     7501 cgaggaggat gatatgagcg acggactccc attcctgaag accgacccca ataacaatgg
     7561 tttctttggc gacggcagcc tgctgtgtat cctgcgaagt attggtttta agccggaaaa
     7621 ttacacgaac agtaatgtca atcgtcttcc cacaatgatc accgaccgtt acacgctggc
     7681 cagtcgcagt actacaagcc gcctgctgca aagctatctt aacaacttcc acccctactg
     7741 ccccatagtg catagtccta ctctcatgat gctttacaac aatcagattg agatagcatc
     7801 gaaggaccag tggcagatcc tcttcaactg cattctcgcc atcggcgctt ggtgtattga
     7861 aggcgagagc acagatatcg acgtgtttta ctatcaaaac gccaagtcgc acctcacctc
     7921 gaaggtcttc gaaagtggaa gcattatcct cgtgacagcc ctccatctgc tgagtcgtta
     7981 tacccagtgg cgtcagaaaa ctaatacatc ctacaacttt cattcgttca gcattcggat
     8041 ggcgattagc ctgggactta atcgtgacct cccaagtagc ttcagcgatt cgtcgatcct
     8101 ggaacagcgc cggaggattt ggtggagtgt gtacagctgg gagatccagc tgtccctctt
     8161 gtacggacgc agcattcagc tgagtcagaa cacgatttcc ttcccgtcca gcgtggacga
     8221 cgttcaacgg acaactacgg gcccgaccat ctaccacggt atcattgaga cggcccgctt
     8281 gcttcaagtg tttacgaaga tttatgagct ggataagaca gtgaccgcgg agaagagtcc
     8341 gatctgcgcc aagaaatgcc tgatgatctg caatgagata gaggaggtga gccgccaggc
     8401 acccaagttc ctgcagatgg acataagcac tacggcgctg actaatctcc tcaaagaaca
     8461 cccctggctg agtttcaccc gcttcgagct gaagtggaaa cagctgagtc tgattatata
     8521 cgttttgcgg gactttttca caaattttac gcagaagaag tcgcagcttg agcaagatca
     8581 gaacgaccat cagtcctacg aggtaaagag gtgcagcata atgctctccg acgcagccca
     8641 gcgcaccgtg atgtcggtgt cgtcctatat ggacaaccat aatgtgaccc cttatttcgc
     8701 ctggaattgc tcctactatt tgttcaacgc cgtgttggtc ccaatcaaga ctcttctgag
     8761 caactccaaa tcgaacgccg agaataacga gacagcacag ctcttgcagc aaattaacac
     8821 cgtgttgatg ctgttgaaga aactcgctac attcaagatt cagacttgcg aaaagtacat
     8881 ccaggtgttg gaagaggtgt gcgccccatt ccttctttcg caatgcgcaa ttcccctccc
     8941 gcacataagt tacaataatt ccaacggctc cgctatcaag aatatcgtcg gctccgctac
     9001 aatcgcgcag tatcccacgc tgcctgagga aaatgttaac aacatatcgg tgaagtatgt
     9061 aagtccaggc agcgtcggcc cgagccctgt gccgctgaaa agcggagcct ccttcagcga
     9121 tctggtcaag ctgctcagca accgcccacc atcccgcaac tcccctgtca ctataccccg
     9181 atcgaccccg tcgcatcgta gcgttacccc attcctggga caacagcagc aactgcagag
     9241 tctggtgcca cttacgcgtc agttggagct ccctcctacc gccacggcta cggcctcgat
     9301 aatgccgcac gtgatggagc agcctctcag taccagcatt aaccccgtca acgaccgctt
     9361 caacggtatt cccaacccca ctccgtataa ctccgatgca gctctcgatg ctatcactca
     9421 gaccaacgat tatggaagcg taaatacaca tggtatcctc tctacttacc cgccaccggc
     9481 tacgcacctt aatgaagctt ccgtcgctct cgctcccggt ggcgcccccc cccgaccgcc
     9541 tcctccgtat gttgacagca cgaccaatca cccgccgtac cactcgaatc tggttccaat
     9601 ggcgaacttt ggttactcga ccgttgatta cgatgccatg gttgacgatt tggctagcat
     9661 tgaatacacg gacgctgtgg atgtcgaccc acagtttatg accaatctgg gattcgttcc
     9721 tggatgtaac ttctccgaca ttaatacata cgaacagtga tgacgtctat cgataccgtc
     9781 gactaaagcc aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat
     9841 ttatttggtt gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt
     9901 aagttagcaa caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt
     9961 tatttaaaga taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag
    10021 atccgcggcc gcggctcgag ggtacctcta gagatccact agtgtcgacg atgtaggtca
    10081 cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
    10141 ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg
    10201 cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg
    10261 tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt
    10321 tctcgacggt cacggcgggc atgtcgacac tagttctagc cagcttttgt tccctttagt
    10381 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10441 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11221 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    11281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12541 catttccccg aaaagtgcca c
//
