LOCUS       sequence-65030-e        9915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pGMR-QF#7
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4706..4724
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     promoter        5040..5278
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5225..5246
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    6452..6881
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     misc_feature    join(6923..7124,7125..7155)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7692..7711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7811..7833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7871..7889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7957..8061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8062..8130,8131..8922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8280..8299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9093..9681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9582..9601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtact tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatgtccag tgcagtaaaa aataaaaaaa aaatatgttt
     4681 ttttaaatct acattctcca aaaaagggtt ttattaactt acatacatac tagaattgat
     4741 ccccgatccc cctagaatcc caaaacaaac tggttattgt ggtaggtcat ttgtttggca
     4801 gaaagaaaac tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag cccagtggaa
     4861 acccttgaaa tgcctttaag tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag
     4921 cccagtggaa acccttgaaa tgcctttaag tcgagcccag tggaaaccct tgaaatgcct
     4981 ttaagtcgac tctagaggat ccgcggccgc gggtaccggg ggatccggcg aaaagagcgc
     5041 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
     5101 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
     5161 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
     5221 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa
     5281 tagggaattc aacatgccac ccaagcgcaa aacgcttaac gctgcggctg aggctaacgc
     5341 tcatgccgac ggacacgccg acggaaacgc cgacggacac gtggccaata cggccgcgtc
     5401 ctcgaataat gcgaggttcg ctgatctcac taacatcgat actccgggtc tgggacccac
     5461 aactacgacc ctgctcgtgg aaccagcacg ctcaaagcgt caacgagtgt cccgcgcatg
     5521 cgaccagtgc cgtgcagccc gagagaaatg cgacggaata cagcctgcgt gtttcccgtg
     5581 cgtttcccag ggaaggtcct gcacttatca ggcttcgccg aaaaagaggg gagttcaaac
     5641 cggttatatt cgtacgctgg agctcgccct cgcctggatg tttgaaaatg tcgcgcgttc
     5701 cgaagatgcc ttgcataacc tcctcgtccg tgacgccgga caaggatcag ctctgctcgt
     5761 tggtaaagat tcgccggctg ccgagcgact ccatgcccgt tgggctacta gccgtgtcaa
     5821 taagagcatt acccgcctcc tccgtcagtt ggagctccct cctaccgcca cggctacggc
     5881 ctcgataatg ccgcacgtga tggagcagcc tctcagtacc agcattaacc ccgtcaacga
     5941 ccgcttcaac ggtattccca accccactcc gtataactcc gatgcagctc tcgatgctat
     6001 cactcagacc aacgattatg gaagcgtaaa tacacatggt atcctctcta cttacccgcc
     6061 accggctacg caccttaatg aagcttccgt cgctctcgct cccggtggcg cccccccccg
     6121 accgcctcct ccgtatgttg acagcacgac caatcacccg ccgtaccact cgaatctggt
     6181 tccaatggcg aactttggtt actcgaccgt tgattacgat gccatggttg acgatttggc
     6241 tagcattgaa tacacggacg ctgtggatgt cgacccacag tttatgacca atctgggatt
     6301 cgttcctgga tgtaacttct ccgacattaa tacatacgaa cagtgagaat tcgttaacag
     6361 atctgcggcc gcggtctaga aggcctaatt ccagctgagc gccggtcgct accattacca
     6421 gttggtctgg tgtcggggat ccgtcgacta aggccaaaga gtctaatttt tgttcatcaa
     6481 tgggttataa catatgggtt atattataag tttgttttaa gtttttgaga ctgataagaa
     6541 tgtttcgatc gaatattcca tagaacaaca atagtattac ctaattacca agtcttaatt
     6601 tagcaaaaat gttattgctt atagaaaaaa taaattattt atttgaaatt taaagtcaac
     6661 ttgtcattta atgtcttgta gacttttgaa agtcttacga tacaattagt atctaatata
     6721 catgggttca ttctacattc tatattagtg atgatttctt tagctagtaa tacattttaa
     6781 ttatattcgg ctttgatgat tttctgattt tttccgaacg gattttcgta gaccctttcg
     6841 atctcataat ggctcatttt attgcgatgg acggtcagga gagctcgaat taacggggat
     6901 ccgtcgacct gcagccaagc tttgcgtact cgcaaattat taaaaataaa actttaaaaa
     6961 taatttcgtc taattaatat tatgagttaa ttcaaacccc acggacatgc taagggttaa
     7021 tcaacaatca tatcgctgtc tcactcagac tcaatacgac actcagaata ctattccttt
     7081 cactcgcact tattgcaagc atacgttaag tggatgtctc ttgccgacgg gaccacctta
     7141 tgttatttca tcatggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg
     7201 ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt
     7261 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     7321 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     7381 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     7441 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
     7501 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
     7561 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
     7621 ccttggagcg actgtcgtta gttccgcgcg attcggtgcg gtatttcaca ccgcatatgg
     7681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7741 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7801 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7861 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7921 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8281 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     8341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     9241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9481 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9661 ggggggcgga gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac
     9721 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
     9781 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
     9841 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
     9901 tacgtgggtc tggcc
//
