LOCUS       sequence-65035-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     Yeast Secretion Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS314-4M5.3
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(3..59,60..257)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVQLDKR"
     CDS             264..287
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgagatt tccttcaatt tttactgcag ttttattcgc agcatcctcc gcattagctg
       61 ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa gctgtcatcg
      121 gttacttaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc aacagcacaa
      181 ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa gaagaagggg
      241 tacagctgga taaaagactc tttgactaca aggacgacga tgacaaggct agcgacgtcg
      301 ttatgactca aacaccacta tcacttcctg ttagtctagg tgatcaagcc tccatctctt
      361 gcagatctag tcagagcctc gtacacagta atggaaacac ctatttacgt tggtacctgc
      421 agaagccagg ccagtctcca aaggtcctga tctacaaagt ttccaaccga gtttctgggg
      481 tcccagacag gttcagtggc agtggatcag ggacagattt cacactcaag atcaacagag
      541 tggaggctga ggatctggga gtttatttct gctctcaaag tacacatgtt ccgtggacgt
      601 tcggtggagg caccaagctt gaaattaagt cctctgctga tgatgctaag aaggatgctg
      661 ctaagaagga tgatgctaag aaagatgatg ctaagaaaga tggtggcgtc aaactggatg
      721 agactggagg aggcttggtg caacctgggg gggccatgaa actctcctgt gttacctctg
      781 gattcacttt tggtcactac tggatgaact gggtccgcca gtctccagag aaaggactgg
      841 agtgggtagc acaatttaga aacaaacctt ataattatga aacatattat tcagattctg
      901 tgaaaggcag attcaccatc tcaagagatg attccaaaag tagtgtctat ctgcaaatga
      961 acaactta
//
