LOCUS       sequence-65038-e        9300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pPTQF#7-hsp70
FEATURES             Location/Qualifiers
     source          1..9300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(229..259,260..814)
                     /label=P element 5' end
     gene            complement(825..4961)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(1592..2206,2277..2408,2629..2944,3006..3660,
                     3735..4008,4403..4474))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4934..4952
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(6536..6737,6738..6768)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7305..7324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7424..7446
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7484..7502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7570..7674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7675..7743,7744..8535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7893..7912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8706..9294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9195..9214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
       61 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
      121 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
      181 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccca tgatgaaata
      241 acataaggtg gtcccgtcga tagccgaagc ttaccgaagt atacacttaa attcagtgca
      301 cgtttgcttg ttgagaggaa aggttgtgtg cggacgaatt tttttttgaa aacattaacc
      361 cttacgtgga ataaaaaaaa atgaaatatt gcaaattttg ctgcaaagct gtgactggag
      421 taaaattaat tcacgtgccg aagtgtgcta ttaagagaaa attgtgggag cagagccttg
      481 ggtgcagcct tggtgaaaac tcccaaattt gtgataccca ctttaaagat tcgcagtgga
      541 aggctgcacc tgcaaaaggt cagacattta aaaggaggcg actcaacgca gatgccgtac
      601 ctagtaaagt gatagagcct gaaccagaaa agataaaaga aggctatacc agtgggagta
      661 cacaaacaga gtaagtttga atagtaaaaa aaatcattta tgtaaacaat aacgtgactg
      721 tgcgttaggt cctgttcatt gtttaatgaa aataagagct tgagggaaaa aattcgtact
      781 ttggagtacg aaatgcgtcg tttagagcag cagccgaatt aattctagtt ccagtgaaat
      841 ccaagcattt tctaaattaa atgtattctt attattatag ttgttatttt tgatatatat
      901 aaacaacact attatgccca ccattttttt gagatgcatc tacacaagga acaaacactg
      961 gatgtcactt tcagttcaaa ttgtaacgct aatcactccg aacaggtcac aaaaaattac
     1021 cttaaaaagt cataatatta aattagaata aatatagctg tgagggaaat atatacaaat
     1081 atattggagc aaataaattg tacatacaaa tatttattac taatttctat tgagacgaaa
     1141 tgaaccactc ggaaccattt gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca
     1201 aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg tgggagacag cgaaagagca
     1261 actacgaaac gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat
     1321 gtatataaaa aatatatccc ggtgttttat gtagcgataa acgagttttt gatgtaaggt
     1381 atgcaggtgt gtaagtcttt tggttagaag acaaatccaa agtctacttg tggggatgtt
     1441 cgaaggggaa atacttgtat tctataggtc atatcttgtt tttattggca caaatataat
     1501 tacattagct ttttgagggg gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa
     1561 aaaacaagca gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta
     1621 gagccagata tgcgagcacc cggaagctca cgatgagaat ggccagaccc acgtagtcca
     1681 gcggcagatc ggcggcggag aagttaagcg tctccaggat gaccttgccc gaactggggc
     1741 acgtggtgtt cgacgatgtg cagctaattt cgcccggctc cacgtccgcc cattggttaa
     1801 tcagcagacc ctcgttggcg taacggaacc atgagaggta cgacaaccat ttgaggtata
     1861 ctggcaccga gcccgagttc aagaagaagc cgccaaagag caggaatggt atgataaccg
     1921 gcggacccac agacagcgcc atcgaggtcg aggagctggc gcaggatatt agatatccga
     1981 aggacgttga cacattggcc accagagtga ccagcgccag gcagttgaag aagtgcagca
     2041 ctccggcccg cagtccgatc atcggatagg caatcgccgt gaagaccagt ggcactgtga
     2101 gaaaaagcgg taattcggca atcgttttgc ccagaaagta tgtgtcacag cgataaagtc
     2161 gacttcgggc ctccctcata aaaactggca gctctgaggt gaacacctaa atcgaatcga
     2221 ttcattagaa agttagtaaa ttattaatat gcaaatgtat tctaaacaag acttacattt
     2281 atcgtggcaa agacgttttg aaaggtcatg ttggtcagga agaggaagat ggctccgttg
     2341 atattcatca cgcccacttg cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg
     2401 gcaaccatct gcaaattaaa atgttactcg catctcatta atattcatat cttcaacatg
     2461 ttcgcgagtt aaatgaaatt tatttatttt ctgcaaaact ataaactata catctcattg
     2521 aaaaaaacta agaagggtgt ggaatcaggc aattctaact aaaatctagc gaatttgttt
     2581 ccaagaattg taagcgttat atcatttgtt tccactggaa ccactcaccg ttgtctgaat
     2641 aagtcgcact tttacgagga gtggttcctt gagcaccgac agccaggatc gccacaggac
     2701 cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac ccattctccg gctgctccag
     2761 tggcttctcc aaatttttgg tggccaacaa ctgctccata tcccgggcta ctttgctaat
     2821 agcaaaattg tcgcatatct tggcgatccg atcacgggac tcgatctccc gtccgggcac
     2881 aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac actgggcacc
     2941 cacgctggat aggagttgag atgtaatgta atgctagata cccttaataa acacatcgaa
     3001 ctcactagga aaagaagtcg acggcttcgc tgggagtgcc caagaaagct accctgccct
     3061 cggccatcag aaggatcttg tcaaagagct caaacagctc ggaagacggc tgatgaatgg
     3121 tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag cacctggacg acgctgtggg
     3181 cggtaaatga gtccagtccg gaggtgggct catcgcagat cagaagcggc ggatcggtta
     3241 gtgcctcgga ggcgaatgcc agacgcttcc tttctccgcc ggacagacct ttcaccctgc
     3301 cgggcacacc gatgatcgtg tgctgacatt tgctgagcga aagctcctgg atcacctgat
     3361 ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg catccgcacc atggcctgga
     3421 aaatcaggtg ttccctggcc gttagggagc cgataaagag gtcatcctgc tggacatagg
     3481 cgcacctggc ctgcatctcc ttggcgtcca caggttggcc attgagcagt cgcatcccgg
     3541 atggcgatac ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct
     3601 ttccggcacc ggaactgccc atcacggcca aaagttcgcc cggataggcc acgccgcaaa
     3661 ctgagtttca aattggtaat tggacccttt attaagattt cacacagatc agccgactgc
     3721 gaatagaaac tcaccgttct tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt
     3781 gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg gctgattgac
     3841 cgccccaaag atgtccatat tgtgccaggc ataggtgagg ttctcggcta gttggccgct
     3901 ccctgaaccg gagtcctccg gcggactggg tggcaggagc gtgccgtagt ttttggcctg
     3961 cccgaagccc tggttaatgc agctctgcga agccgctccg ctgtcaccct gcaatgatag
     4021 gggatctcaa atatcaacta caagcgttat gctcatctaa ccccgaacaa aacgaagtat
     4081 cctacgaagt aggtttatac ttttatttat tttttgtgca tctaggatca gcttaaaata
     4141 tctggttgtt atattttttg taaaaaagaa tgtagtcgaa aatgaatgcc tttagatgtc
     4201 ttgatcatga tatgatctta aaaattgtct tatatagcga gcacagctac cagaataatc
     4261 tgtttcgtgt cactatttgt ttgtgcgatt gcggtttggg atttttgtgg gtcgcagttc
     4321 tcacgccgca gacaatttga tgttgcaatc gcagttccta tagatcaagt gaacttaaga
     4381 tgtatgcaca tgtactactc acattgttca gatgctcggc agatgggtgt ttgctgcctc
     4441 cgcgaattaa tagctcctga tcctcttggc ccattgccgg gatttttcac actttcccct
     4501 gcttacccac ccaaaaccaa tcaccacccc aatcactcaa aaaacaaaca aaaataagaa
     4561 gcgagaggag ttttggcaca gcactttgtg tttaattgat ggcgtaaacc gcttggagct
     4621 tcgtcacgaa accgctgaca aagtgcaact gaaggcggac attgacgcta ggtaacgcta
     4681 caaacggtgg cgaaagagat agcggacgca gcggcgaaag agacggcgat atttctgtgg
     4741 acagagaagg aggcaaacag cgctgacttt gagtggaatg tcattttgag tgagaggtaa
     4801 tcgaaagaac ctggtacttc aaataccctt ggatcgaagt aaatttaaaa ctgatcagat
     4861 aagttcaatg atatccagtg cagtaaaaaa taaaaaaaaa atatgttttt ttaaatctac
     4921 attctccaaa aaagggtttt attaacttac atacatacta gaattaggcc ttctagaccg
     4981 cggccgcaga tctgttaacg aattcggtac cagatccggg atccggcgaa aagagcgccg
     5041 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     5101 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     5161 tgcaggaatt ccaacatgcc acccaagcgc aaaacgctta acgctgcggc tgaggctaac
     5221 gctcatgccg acggacacgc cgacggaaac gccgacggac acgtggccaa tacggccgcg
     5281 tcctcgaata atgcgaggtt cgctgatctc actaacatcg atactccggg tctgggaccc
     5341 acaactacga ccctgctcgt ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca
     5401 tgcgaccagt gccgtgcagc ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg
     5461 tgcgtttccc agggaaggtc ctgcacttat caggcttcgc cgaaaaagag gggagttcaa
     5521 accggttata ttcgtacgct ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt
     5581 tccgaagatg ccttgcataa cctcctcgtc cgtgacgccg gacaaggatc agctctgctc
     5641 gttggtaaag attcgccggc tgccgagcga ctccatgccc gttgggctac tagccgtgtc
     5701 aataagagca ttacccgcct cctccgtcag ttggagctcc ctcctaccgc cacggctacg
     5761 gcctcgataa tgccgcacgt gatggagcag cctctcagta ccagcattaa ccccgtcaac
     5821 gaccgcttca acggtattcc caaccccact ccgtataact ccgatgcagc tctcgatgct
     5881 atcactcaga ccaacgatta tggaagcgta aatacacatg gtatcctctc tacttacccg
     5941 ccaccggcta cgcaccttaa tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc
     6001 cgaccgcctc ctccgtatgt tgacagcacg accaatcacc cgccgtacca ctcgaatctg
     6061 gttccaatgg cgaactttgg ttactcgacc gttgattacg atgccatggt tgacgatttg
     6121 gctagcattg aatacacgga cgctgtggat gtcgacccac agtttatgac caatctggga
     6181 ttcgttcctg gatgtaactt ctccgacatt aatacatacg aacagtgatg acgtctatcg
     6241 ataccgtcga ctaaagccaa atagaaaatt attcagttcc tggcttaagt ttttaaaagt
     6301 gatattattt atttggttgt aaccaaccaa aagaatgtaa ataactaata cataattatg
     6361 ttagttttaa gttagcaaca aattgatttt agctatatta gctacttggt taataaatag
     6421 aatatattta tttaaagata attgcgtttt tattgtcagg gagtgagttt gcttaaaaac
     6481 tcgtttagat ccgcggccgc ggctcgaggg tacctctaga ctgcagccca agctttgcgt
     6541 actcgcaaat tattaaaaat aaaactttaa aaataatttc gtctaattaa tattatgagt
     6601 taattcaaac cccacggaca tgctaagggt taatcaacaa tcatatcgct gtctcactca
     6661 gactcaatac gacactcaga atactattcc tttcactcgc acttattgca agcatacgtt
     6721 aagtggatgt ctcttgccga cgggaccacc ttatgttatt tcatcatggt ctggccattc
     6781 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     6841 agtagccgac atatatccga aataactgct tgtttttttt tttaccatta ttaccatcgt
     6901 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     6961 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     7021 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     7081 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     7141 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     7201 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     7261 gcgattcggt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     7321 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7381 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7441 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7501 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8821 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     8881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9061 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     9121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
//
