LOCUS       sequence-65039-e       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pQUAST-GAL80
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3503..4810
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     intron          4898..4963
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5093..5113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5385..5519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5422..5441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5476..5495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            5537..9664
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(5546..5564)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6015..6086,6481..6754,6829..7483,7545..7860,8081..8212,
                     8283..8897)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9675..10229,10230..10260)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc ggctcgagca aaatggacta caacaagaga tcttcggtct caaccgtgcc
     3541 taatgcagct cccataagag tcggattcgt cggtctcaac gcagccaaag gatgggcaat
     3601 caagacacat taccccgcca tactgcaact atcgtcacaa tttcaaatca ctgccttata
     3661 cagtccaaaa attgagactt ctattgccac cattcagcgt ctaaaattga gtaatgccac
     3721 tgcttttccc actttagagt catttgcatc atcttccact atagatatga tagtgatagc
     3781 tatccaagtg gccagccatt atgaagttgt tatgcctctc ttggaattct ccaaaaataa
     3841 tccgaacctc aagtatcttt tcgtagaatg ggcccttgca tgttcactag atcaagccga
     3901 atccatttat aaggctgctg ctgaacgtgg ggttcaaacc atcatctctt tacaaggtcg
     3961 taaatcacca tatattttga gagcaaaaga attaatatct caaggctata tcggcgacat
     4021 taattcgatc gagattgctg gaaatggcgg ttggtacggc tacgaaaggc ctgttaaatc
     4081 accaaaatac atctatgaaa tcgggaacgg tgtagatctg gtaaccacaa catttggtca
     4141 cacaatcgat attttacaat acatgacaag ttcgtacttt tccaggataa atgcaatggt
     4201 tttcaataat attccagagc aagagctgat agatgagcgt ggtaaccgat tgggccagcg
     4261 agtcccaaag acagtaccgg atcatctttt attccaaggc acattgttaa atggcaatgt
     4321 tccagtgtca tgcagtttca aaggtggcaa acctaccaaa aaatttacca aaaatttggt
     4381 cattgacatt cacggtacca agggagattt gaaacttgaa ggcgatgccg gcttcgcaga
     4441 aatttcaaat ctggtccttt actacagtgg aactagagca aacgacttcc cgctagccaa
     4501 tggacaacaa gctcctttag acccggggta tgatgcaggt aaagaaatca tggaagtata
     4561 tcatttacga aattataatg ccattgtggg taatattcat cgactgtatc aatctatctc
     4621 tgacttccac ttcaatacaa agaaaattcc tgaattaccc tcacaatttg taatgcaagg
     4681 tttcgatttc gaaggctttc ccaccttgat ggatgctctg atattacaca ggttaatcga
     4741 gagcgtttat aaaagtaaca tgatgggctc cacattaaac gttagcaata tctcgcatta
     4801 tagtttataa gacgtctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     4861 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     4921 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     4981 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     5041 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     5101 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     5161 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     5221 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     5281 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5341 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     5401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     5521 actagaaggc ctaattctag tatgtatgta agttaataaa acccattttt gcggaaagta
     5581 gataaaaaaa acattttttt tttttactgc actggatatc attgaactta tctgatcagt
     5641 tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat tacctctcac
     5701 tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc cacagaaata
     5761 tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt agcgttacgt
     5821 agcgtcaatg tccgccttca gttgcttttg tcagcggttt cgtgacgaag ctccaagcgg
     5881 tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg
     5941 tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt
     6001 gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac
     6061 acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac
     6121 ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc
     6181 acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga ttattctggt
     6241 agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga catctaaagg
     6301 cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata ttttaagctg
     6361 atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcgtttt
     6421 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     6481 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     6541 tacggcacgc tcctgccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     6601 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     6661 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     6721 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     6781 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     6841 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     6901 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     6961 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     7021 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg
     7081 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     7141 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     7201 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc
     7261 cgcttctgat ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg
     7321 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     7381 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     7441 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     7501 attaagggta tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt
     7561 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     7621 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgctat tagcaaagta
     7681 gcccgggata tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg
     7741 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     7801 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     7861 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     7921 tagattttag ttagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     7981 tagtttatag ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca tgttgaagat
     8041 atgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     8101 cctcatcttt ttgggccaac aactcacgca agtgggcgtg atgaatatca acggagccat
     8161 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtct
     8221 tgtttagaat acatttgcat attaataatt tactaacttt ctaatgaatc gattcgattt
     8281 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     8341 gtgacacata ctttctgggc aaaacgattg ccgaattacc gctttttctc acagtgccac
     8401 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     8461 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     8521 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     8581 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     8641 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     8701 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     8761 gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg
     8821 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     8881 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     8941 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     9001 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     9061 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     9121 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     9181 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     9241 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     9301 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
     9361 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
     9421 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
     9481 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
     9541 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
     9601 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
     9661 ctagaattaa ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt
     9721 ttccctcaag ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat
     9781 tgtttacata aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg
     9841 tatagccttc ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg
     9901 cgttgagtcg cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat
     9961 cattaaagtg ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct
    10021 cccacaattt tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag
    10081 ctttgcagca aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt
    10141 caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt
    10201 aagtgtatac ttcggtaagc ttcggctatc gacgggacca ccttatgtta tttcatcatg
//
