LOCUS       sequence-65045-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Seifert HS
  TITLE     Insertionally inactivated and inducible recA alleles for use in 
            Neisseria.
  JOURNAL   Gene. 1997 Apr 1;188(2):215-20.
  PUBMED    9133594
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1997-04-1"; volume: "188(2)"; pages: "215-20"
COMMENT     Sequence Label: pVD300recA6
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(313..329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(313..329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(361..367,368..385,386..391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    421..437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(445..451,452..467,468..473))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        703..780
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(800..819)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ggcgcacacg ccgccgtttt tctggcattg ggcgacggct tcgaggcaga gtgtggtttt
       61 gccggaggat tcggggccga agatttcgac gatgcgcccg cgcggcaggc cgccgactcc
      121 gagggcgagg tctaatccga gcgatccggt ggaaatgact tcgaggtttt cttcctgctg
      181 gctgccgtcc attttcatga tggcgccttt gccgaaactt ttttcgattt gcgccagtgc
      241 ggcggcaagg gctttgcttt tgtcgtctga catgggggtt actccggagc ggccgcggaa
      301 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctagtttc ctgtgtgaaa
      421 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt caacagctca
      481 tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
      541 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
      601 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat aagcccggat
      661 cagcttgcaa ttcgcgcgcg aaggcgaagc ggcatttacg ttgacaccat cgaatggtgc
      721 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
      781 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctc
//
