LOCUS       sequence-65047-e       11285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of murine CD8GFP in insects.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Riabinina O, Task D, Marr E, Lin CC, Alford R, O'Brochta DA, Potter 
            CJ
  TITLE     Organization of olfactory centres in the malaria mosquito Anopheles 
            gambiae.
  JOURNAL   Nat Commun. 2016 Oct 3;7:13010. doi: 10.1038/ncomms13010.
  PUBMED    27694947
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-3"; volume: "7:13010. doi"; pages: " 
            10.1038/ncomms13010"
COMMENT     Sequence Label: pQUASp-mCD8GFP
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(1986..2016,2017..2571)
                     /label=P element 5' end
     CDS             complement(join(3350..3964,4035..4166,4370..4685,4747..5401,
                     5476..5749,6151..6222))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6674..6692
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             7398..8063
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             8070..8783
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8100..8128)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8723..8743
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(10276..10477,10478..10508)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(11047..11066)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11166..11188
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11226..11244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     1801 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     1861 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     1921 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     1981 tggcccatga tgaaataaca taaggtggtc ccgtcgatag ccgaagctta ccgaagtata
     2041 cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg ttgtgtgcgg acgaattttt
     2101 ttttgaaaac attaaccctt acgtggaata aaaaaaaatg aaatattgca aattttgctg
     2161 caaagctgtg actggagtaa aattaattca cgtgccgaag tgtgctatta agagaaaatt
     2221 gtgggagcag agccttgggt gcagccttgg tgaaaactcc caaatttgtg atacccactt
     2281 taatgattcg cagtggaagg ctgcacctgc aaaaggtcag acatttaaaa ggaggcgact
     2341 caacgcagat gccgtaccta gtaaagtgat agagcctgaa ccagaaaaga taaaagaagg
     2401 ctataccagt gggagtacac aaacagagta agtttgaata gtaaaaaaaa tcatttatgt
     2461 aaacaataac gtgactgtgc gttaggtcct gttcattgtt taatgaaaat aagagcttga
     2521 gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt agagcagcag ccgaattaat
     2581 tctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
     2641 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
     2701 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
     2761 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
     2821 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
     2881 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
     2941 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
     3001 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
     3061 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
     3121 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
     3181 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
     3241 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
     3301 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
     3361 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
     3421 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
     3481 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
     3541 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
     3601 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
     3661 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
     3721 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
     3781 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
     3841 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
     3901 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
     3961 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
     4021 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
     4081 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
     4141 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
     4201 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
     4261 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
     4321 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
     4381 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
     4441 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
     4501 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
     4561 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
     4621 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
     4681 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
     4741 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
     4801 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
     4861 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
     4921 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
     4981 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
     5041 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
     5101 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
     5161 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
     5221 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
     5281 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
     5341 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
     5401 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
     5461 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
     5521 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
     5581 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
     5641 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
     5701 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
     5761 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaaagtaccc
     5821 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
     5881 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
     5941 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
     6001 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
     6061 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
     6121 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
     6181 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
     6241 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
     6301 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
     6361 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
     6421 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
     6481 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
     6541 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
     6601 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
     6661 tttccgcaaa aatgggtttt attaacttac atacatacta gaattggccg ctctagcccc
     6721 ccctcgaatg ttctctctct tctcttctct ctctctttct cgaatgttct ctctcttctc
     6781 ttctctctct ctttctcgag tcacgtgtca ctgggtcagt gggtaatcgc ttatcctcgg
     6841 ataaacaatt atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt
     6901 aatcgcttat ccttgggtaa tcgcttatcc tcggataaac aattatcctc acgggtaatc
     6961 gcttatccgc tcgggtaatc gcttatcctc gggtaatcgc ttatccttgg gtaatcgctt
     7021 atcctcggat aaacaattat cctcacgggt aatcgcttat ccgctcgggt aatcgcttat
     7081 cctcgggtaa tcgcttatcc tttcacgttg ggactcagtg actcgatagc cgaagcttac
     7141 cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt tgtgtgcgga
     7201 cgaatttttt tttgaaaacc ggtgatagag cctgaaccag aaaagataaa agaaggctat
     7261 accagtggga gtacacaaac agagtaagtt tgaatagtaa aaaaaatcat ttatgtaaac
     7321 aataacgtga ctgtgcgtta ggtcctgttc attggtaccc gcccggggat cagatccgcg
     7381 gccgcggctc gagcaaaatg gcctcaccgt tgacccgctt tctgtcgctg aacctgctgc
     7441 tgctgggtga gtcgattatc ctggggagtg gagaagctaa gccacaggca cccgaactcc
     7501 gaatctttcc aaagaaaatg gacgccgaac ttggtcagaa ggtggacctg gtatgtgaag
     7561 tgttggggtc cgtttcgcaa ggatgctctt ggctcttcca gaactccagc tccaaactcc
     7621 cccagcccac cttcgttgtc tatatggctt catcccacaa caagataacg tgggacgaga
     7681 agctgaattc gtcgaaactg ttttctgcca tgagggacac gaataataag tacgttctca
     7741 ccctgaacaa gttcagcaag gaaaacgaag gctactattt ctgctcagtc atcagcaact
     7801 cggtgatgta cttcagttct gtcgtgccag tccttcagaa agtgaactct actactacca
     7861 agccagtgct gcgaactccc tcacctgtgc accctaccgg gacatctcag ccccagagac
     7921 cagaagattg tcggccccgt ggctcagtga aggggaccgg attggacttc gcctgtgata
     7981 tttacatctg ggcacccttg gccggaatct gcgtggccct tctgctgtcc ttgatcatca
     8041 ctctcatctg ctaccacagc cgcggatcca tgagtaaagg agaagaactt ttcactggag
     8101 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     8161 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     8221 gaaaactacc tgttccatgg ccaacacttg tcactacttt aacttatggt gttcaatgct
     8281 tttcaagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     8341 gttatgtcca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     8401 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     8461 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     8521 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     8581 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     8641 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     8701 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     8761 atggcatgga tgaactatac aaataatcta gagtcgacct cgaacgttaa cgttaacgta
     8821 acgttaactc gaggagcttg ataacattat acctaaaccc atggtcaaga gtaaacattt
     8881 ctgcctttga agttgagaac acaattaagc atcccctggt taaacctgac attcatactt
     8941 gttaatagcg ccataaacat agcaccaatt cgaagaatac ttgttaatag cgccataaac
     9001 atagcaccaa tttcgaagaa actcagttaa aagcaattag caattagcaa ttagcaataa
     9061 ctctgctgac ttcaaaacga gaagagttgc aagtatttgt aaggcacagt ttatagacca
     9121 ccgacggctc attagggctc gtcatgtaac taagcgcggt gaaacccaat tgaacatata
     9181 gtggaattat tattatcaat ggggaagatt taaccctcag gtagcaaagt aatttaattg
     9241 caaatagaga gtcctaagac taaataatat atttaaaaat ctggcccttt gaccttgctt
     9301 gtcaggtgca tttgggttca atcgtaagtt gcttctatat aaacactttc cccatccccg
     9361 caataatgaa gaataccgca gaataaagag agatttgcaa caaaaaataa aggcattgcg
     9421 aaaacttttt atgggggatc attacactcg ggcctacggt tacaattccc agccacttaa
     9481 gcgacaagtt tggccaacaa tccatctaat agctaatagc gcaatcactg gtaatcgcaa
     9541 gagtatatag gcaatagaac ccatggattt gaccaaaggt aaccgagaca atggagaagc
     9601 aagaggattt caaactgaac acccacagtg ctgtgtacta ccactggcgc gtttgggagc
     9661 tcactggcct gatgcgtcct ccgggcgttt caagcctgct ttacgtggta tactccatta
     9721 cggtcaactt ggtggtcacc gtgctgtttc ccttgagctt gctggccagg ctgctgttca
     9781 ccaccaacat ggccggattg tgcgagaacc tgaccataac tattaccgat attgtggcca
     9841 atttgaagtt tgcgaatgtg tacatggtga ggaagcagct ccatgagatt cgctctctcc
     9901 taaggctcat ggacgctaga gcccggctgg tgggcgatcc cgaggagatt tctgccttga
     9961 ggaaggaagt gaatatcgca cagggcactt tccgcacctt tgccagtatt ttcgtatttg
    10021 gcactacttt gagttgcgtc cgcgtggtcg ttcgcccgga tcgagagctc ctgtatccgg
    10081 cctggttcgg cgttgactgg atgcactcca ccagaaacta tgtgctcatc aatatctacc
    10141 agctcttcgg cttgatagtg caggctatac agaactgcgc tagtgactcc tatccgcctg
    10201 cgtttctctg cctgctcacg ggtcatatgc gtgctttgga gctgagggtg cggcggattg
    10261 gctgcagcca agctttgcgt actcgcaaat tattaaaaat aaaactttaa aaataatttc
    10321 gtctaattaa tattatgagt taattcaaac cccacggaca tgctaagggt taatcaacaa
    10381 tcatatcgct gtctcactca gactcaatac gacactcaga atactattcc tttcactcgc
    10441 acttattgca agcatacgtt aagtggatgt ctcttgccga cgggaccacc ttatgttatt
    10501 tcatcatggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag
    10561 acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt
    10621 ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt
    10681 atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac
    10741 atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc
    10801 ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt tatatacata
    10861 cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc
    10921 tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg
    10981 agcgactgtc gttagttccg cgcgattcgg gtgcggtatt tcacaccgca tatggtgcac
    11041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
    11101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    11161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
    11221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    11281 gacgt
//
