LOCUS       sequence-65059-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a luciferase gene disrupted by a QQR ZFN target site and a
            frame-shift mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGreenII Tfs-qqr::LUC
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(132..150)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttcatttgg agaggacagc ccaagctcat gtctagactt cttcccctca ggaggggaag
       61 aactcgagga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag
      121 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa
      181 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa
      241 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
      301 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
      361 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg
      421 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg
      481 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc
      541 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat
      601 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct
      661 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat
      721 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa
      781 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg
      841 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg
      901 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca
      961 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc
     1021 tctctaagga a
//
