LOCUS       sequence-65060-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGreenII 0579-1 (35S::LUC)
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(73..92)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(74..92)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(102..119)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(102..118)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(111..133)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        336..681
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     624..644
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(758..776)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
       61 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      121 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      181 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      241 agcctgaatg gcgaatggaa attgtaagcg ttaatgttat cgtaccccta ctccaaaaat
      301 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt
      361 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      421 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
      481 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      541 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac
      601 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      661 tcatttcatt tggagaggac agcccaagct catggaagac gccaaaaaca taaagaaagg
      721 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
      781 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
      841 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
      901 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
      961 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     1021 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttcc
//
