LOCUS       sequence-65078-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged Elongin B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Mahrour N, Redwine WB, Florens L, Swanson SK, Martin-Brown S, 
            Bradford WD, Staehling-Hampton K, Washburn MP, Conaway RC, Conaway 
            JW
  TITLE     Characterization of Cullin-box sequences that direct recruitment of 
            Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin 
            ligases.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):8005-13. Epub 2008 Jan 10.
  PUBMED    18187417
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-03-21"; volume: "283(12)"; pages: "8005-13. Epub
            2008 Jan 10"
COMMENT     Sequence Label: pcDNA5/FRT-Flag-EloB
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(393..416)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(438..457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(439..457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(502..705)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(531..551)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        706..1010
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gactcgacgc ggccgcgctg caggaattct cactgcacag cttgttcatt ggcactgcct
       61 ccagaatcct gtggcttcat cacatctgga agctccggag ggctggagaa gggttcaata
      121 cgcagcgctt caaaggtgtc atctgctcga aaggccaggc ccactgtggc tggggcctgt
      181 ggccttgctg tctgactggt gaagccacat tcgcccagag tttttccatc atcaaggagc
      241 tggtcgtcct tgtacagccg ctgctcctct ggcggccgct tgaggatgcc ctcgacgatg
      301 cgcttcagtt cgaacaccgt gctcgactcc ttggcgtccg taaagatggt ggtcttgtgg
      361 cgccggatca tgagaaacac gtccatctcg agcttatcgt cgtcatcctt gtaatccatg
      421 ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
      481 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
      541 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
      601 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
      661 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
      721 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
      781 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
      841 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      901 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggccggg
      961 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     1021 tatatgggct atgaactaat gaccccgtat g
//
