LOCUS       sequence-65104-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Yeh E, Cunningham M, Arnold H, Chasse D, Monteith T, Ivaldi G, Hahn 
            WC, Stukenberg PT, Shenolikar S, Uchida T, Counter CM, Nevins JR, 
            Means AR, Sears R
  TITLE     A signalling pathway controlling c-Myc degradation that impacts 
            oncogenic transformation of human cells.
  JOURNAL   Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14.
  PUBMED    15048125
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2004-04"; volume: "6(4)"; pages: "308-18. Epub 2004 
            Mar 14"
COMMENT     Sequence Label: pD40-His/V5-c-MycT58A
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg
      121 tcggcgcgcc caccctttgc accagagttt cgaagctgtt cgagtttgtg tttcaactgt
      181 tctcgtcgtt tcctcaataa gtccttttca gaggtgagct tgtgctcgtc tgcttgaatg
      241 gacaggatgt aggcggtggc ttttttgagg atcactacct tgggggcctt ttcgttgttt
      301 tccaattcag ggatctggtc acgcagggca aaaaagctgc gcttcagctc gttcctcctc
      361 tgacgttcca agacgttgtg tgtccgcctc ttgtcgtttt cctccgtgtc tgaggacctg
      421 gggctggagc acttgcggtt gttgctgatc tgcttcagga ccctgccact gtccaacttg
      481 gccctcttgg cagctggata gtccttcctt gtggaggggg gtgcggcgta gttgtgctgg
      541 tgagtggaga cgtggcacct cttgaggacc agtgggctgt gcggaggttt gctgtggcct
      601 cgggatggag atgagcccga ctccgacctc ttggcagggg tttgcctctt ctccacagac
      661 accacatcaa tttcttcctc atcttcttgc tcttcttcag agtcgctgct ggtggtgggc
      721 ggtgtctcct catgcagcac taggggctca gggctggccc gtggggagga ctcggaggac
      781 agcagcgagt ccgaggaagg agagaaggcc gtggaatcgg acgaggtaca ggatttgggc
      841 gagctgct
//
