LOCUS       sequence-65105-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Yeh E, Cunningham M, Arnold H, Chasse D, Monteith T, Ivaldi G, Hahn 
            WC, Stukenberg PT, Shenolikar S, Uchida T, Counter CM, Nevins JR, 
            Means AR, Sears R
  TITLE     A signalling pathway controlling c-Myc degradation that impacts 
            oncogenic transformation of human cells.
  JOURNAL   Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14.
  PUBMED    15048125
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2004-04"; volume: "6(4)"; pages: "308-18. Epub 2004 
            Mar 14"
COMMENT     Sequence Label: pD40-His/V5-c-MycS62A
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 ggcgcgccca ccctttgcac cagagtttcg aagctgttcg agtttgtgtt tcaactgttc
      181 tcgtcgtttc ctcaataagt ccttttcaga ggtgagcttg tgctcgtctg cttgaatgga
      241 caggatgtag gcggtggctt ttttgaggat cactaccttg ggggcctttt cgttgttttc
      301 caattcaggg atctggtcac gcagggcaaa aaagctgcgc ttcagctcgt tcctcctctg
      361 acgttccaag acgttgtgtg tccgcctctt gtcgttttcc tccgtgtctg aggacctggg
      421 gctggagcac ttgcggttgt tgctgatctg cttcaggacc ctgccactgt ccaacttggc
      481 cctcttggca gctggatagt ccttccttgt ggaggggggt gcggcgtagt tgtgctggtg
      541 agtggagacg tggcacctct tgaggaccag tgggctgtgc ggaggtttgc tgtggcctcg
      601 ggatggagat gagcccgact ccgacctctt ggcaggggtt tgcctcttct ccacagacac
      661 cacatcaatt tcttcctcat cttcttgctc ttcttcagag tcgctgctgg tggtgggcgg
      721 tgtctcctca tgcagcacta ggggctcagg gctggcccgt ggggaggact cggaggacag
      781 cagcgag
//
