LOCUS       sequence-65107-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A
  TITLE     Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end 
            rule pathway.
  JOURNAL   J Biol Chem. 2008 Aug 29. 283(35):24011-28.
  PUBMED    18566452
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Aug 29. 283(35):24011-28."
COMMENT     Sequence Label: UPR pBAL Ura3
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(216..242)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(381..608)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(636..662)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
       61 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
      121 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
      181 tccttatatg tagctttcga ctggccaagg aaagcggcgt aatctgggac gtcgtatggg
      241 tagccacggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      301 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
      361 caagctccgg atccgtgcag accacctctt agccttagca caagatgtaa ggtggactcc
      421 ttctgaatgt tgtaatcaga cagcgttcta ccgtcttcta gctgcctacc ggcaaagatc
      481 aatctttgtt gatctggagg gataccttcc ttgtcttgaa ttttcgactt aacgttgtcg
      541 atggtatcgg aagattcaac ttccaatgtt atggttttac cggtcaaagt cttgacgaaa
      601 atctgcatgg tcgacccact acccgcaagg aaagcggcgt aatctgggac atcgtatggg
      661 taggtaccgt ctttcttctc gtagacttca aacttatact tgatgccttt ttcctcctgg
      721 acctcagaga ggacgcctgg gtattctggg agaagtttat atttccccaa atcaatttct
      781 gggaaaaacg tgtcactttc aaattcctgc atgatccttg tcacaaagag tctgaggtgg
      841 cctggttgat tcatggcttc ctggtaaaca gaactgcctc cgactatcca aaccatgtct
      901 actttacttg ccaattccgg ttgttcaata agtcttaagg catcatccaa actttt
//
