LOCUS       sequence-65109-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Rouhana L, Weiss JA, King RS, Newmark PA
  TITLE     PIWI homologs mediate histone H4 mRNA localization to planarian 
            chromatoid bodies.
  JOURNAL   Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. 
            Epub 2014 Jun 5.
  PUBMED    24903754
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.101618"; journalName: "Development"; date: "2014-07"; 
            volume: "141"; issue: "13"; pages: "2592-601"
COMMENT     Sequence Label: pBS-Histone H1
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(871..891)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(871..889)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(910..926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(910..926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(923..945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    934..950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaggtcgacg gtatcgataa gcttgatatc gaattccatt gtgttgggta taaagccaaa
       61 agcagatgtg aaaccgaaga gtgatgctaa atcgaagcct gcgaaagcaa aatcaacacc
      121 gaatcatcct ccagctatgg atatgatcaa agcagcgatt aaatcaagcc aggatcgcac
      181 agggatgtcg ttgattttaa ttaaaaaatt tattcaacaa aattataaca tcaatatcga
      241 taagtacggt ccgtttttgc gtcgtgcgct tagtcatgga gttgagaaag gagttttgat
      301 tcgcgttggc aacaaaggaa aaggtgcttc aggaagcttt aagctgccat ctaagaagga
      361 tgccagcaaa acagattcac ttgaaaaggt ctcaaagaag ccagcaccaa aaaagctgat
      421 aaagaaacct atgaagaaaa cagagaaaaa agttgttgct aaaaagccta taaagaaagt
      481 cgaaaagaag gcagaagaga agaaagacga gaaagttgat aaagtcgatg ataagaaagt
      541 agttaagaag tcgcctgtaa aaagacaagc cgaaaaaaga aaatcgataa caccgaaaaa
      601 agttgttaag gttgatgcac ataaatccaa aattgctaaa gctaaagcaa ctccaaaaaa
      661 ggttgctccg aaaaaaactc cgaaaaaagc gcctgttaaa gcaaagaagt aaattttata
      721 attgagaatg ctaacggccc tctttagggc cacatatgtt gattgtgctg taaatgttgt
      781 acataaatgt aaatatattt catggtttaa taaaaaaaaa aaaaaaaaat gcgagcggcc
      841 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
      901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      961 catacgagcc ggaa
//
