LOCUS       sequence-65110-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Rouhana L, Weiss JA, King RS, Newmark PA
  TITLE     PIWI homologs mediate histone H4 mRNA localization to planarian 
            chromatoid bodies.
  JOURNAL   Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. 
            Epub 2014 Jun 5.
  PUBMED    24903754
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.101618"; journalName: "Development"; date: "2014-07"; 
            volume: "141"; issue: "13"; pages: "2592-601"
COMMENT     Sequence Label: pBS-Histone H2B
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..672)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(652..670)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(691..707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(691..707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(704..726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    715..731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(739..745,746..763,764..769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    784..805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(922..939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tccatttgtt gggaagatcg
       61 ctgctaagtc tgttaagaag atttcaaagg aagttactcc taaaacagac aaaaagaaaa
      121 ggatacataa acgcaaagaa agttatggta tttacatcta caaagtgttg agacaagttc
      181 atccagatac tggaatttct ggcaaagcaa tgtctattat gaatagcttt gtcaacgatg
      241 ttttcgaaag aatcgcttcg gaggctagca agttggcaac ttataataag aaatcaacca
      301 taaccagcag agagattcaa actgcagtca ggttaatttt accaggagaa ttggctaaac
      361 acgcagttag tgaaggaacc aaagctgtta caaaatacac aggatccaaa taaaaactat
      421 caataaagag aaagttgaac ggccctcttt agggccacaa ttcttgattg ttttaatgtc
      481 actagttata ctatgtattg ataatgttct gaacttttaa aacgtcgttg aagtacgtat
      541 tatcttttat cttatgtcac gtttattaat gtaaataaat gtttgtattt ctaaaaaaaa
      601 aaaaaaaaaa tgcgagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
      661 agggttaatt gctcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggtgag gcggtttgcg
      901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcg
//
